Chromatin and gene organization during spermatogenesis and early development An evolutionary perspective

精子发生和早期发育过程中的染色质和基因组织进化视角

• 批准号: 46399-2007
• 负责人: Ausió, Juan
• 金额: $ 5.46万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364324
• 关键词: Chromatin; gene; organization; during; spermatogenesis

Spermatogenesis is a cell differentiation process leading to the formation of the male gametes (spermatozoa). The deoxyribonucleic acid (DNA) of sperm cells is associated with a set of highly basic proteins called protamines. This contrasts with the rest of the body, including eggs, in which the DNA is associated with a broad spectrum of nuclear proteins such as core histones, linker histones and non-histone proteins. In both instances, the resulting protein-DNA complexes are called chromatin.    During spermatogenesis, histones of chromatin from the stem cells are replaced in the mature sperm by sperm-specific histones (H), protamines (P), or protamine-like proteins (PL), which have an intermediate chemical composition between H and P. This histone replacement process reverts during fertilization of the egg by the sperm.    The molecular mechanisms involved in these chromatin rearrangements are poorly understood. The long-term objective of this research is to describe and understand the structural changes and factors involved in the chromatin modifications during spermatogenesis and after fertilization. To facilitate this, we have selected several invertebrate and vertebrate organisms, with high levels of gametes, for analysis.    This research has a two-fold interest. From an applied perspective, the understanding of these chromatin transitions could enlighten society on human infertility problems, sperm competition, and speciation in other organisms. From an academic perspective, the analysis and characterization of the genes and corresponding proteins involved in these processes, in different organisms, provide a unique opportunity to study their evolution.

精子发生是一个细胞分化过程，导致形成雄配子（精子）。精子细胞的脱氧核糖核酸（DNA）与一组称为鱼精蛋白的高碱性蛋白质有关。这与身体的其他部分形成对比，包括卵子，其中DNA与广泛的核蛋白质如核心组蛋白，连接组蛋白和非组蛋白蛋白相关联。在这两种情况下，产生的蛋白质-DNA复合物被称为染色质。 在精子发生过程中，来自干细胞的染色质组蛋白在成熟精子中被精子特异性组蛋白（H）、鱼精蛋白（P）或鱼精蛋白样蛋白（PL）取代，这些蛋白具有H和P之间的中间化学组成。 涉及这些染色质重排的分子机制知之甚少。本研究的长期目标是描述和了解精子发生过程中和受精后染色质修饰的结构变化和相关因素。为了促进这一点，我们选择了几种无脊椎动物和脊椎动物的生物体，与高水平的配子，进行分析。 这项研究具有双重意义。从应用的角度来看，对这些染色质转变的理解可以启发社会对人类不育问题，精子竞争和其他生物体的物种形成。从学术的角度来看，在不同的生物体中，对参与这些过程的基因和相应蛋白质的分析和表征，为研究它们的进化提供了一个独特的机会。