Quantitative polymerase chain reaction system

实时（RT）聚合酶链反应（PCR），也称为定量PCR (Q-PCR)，是一种扩增已知核苷酸序列的核酸片段并能够量化原始样品中通常非常少量的核酸的方法。在我的实验室（目前由NSERC资助）进行的研究的几个方面涉及编码蛋白质的基因特征，这些蛋白质参与细胞分化过程（精子发生），导致男性精子细胞（精子）的形成。我们也对卵子受精后立即与精子相互作用的蛋白质及其基因表达的特征非常感兴趣。因此，我们目前的大部分工作涉及到使用Q-PCR分析和表征来自不同脊椎动物和无脊椎动物生物的基因，以及它们表达的时间和数量。除了了解精子发生和早期胚胎发生的基本分子机制外，该研究对不育具有重要意义。在我们的实验室，我们过去一直广泛使用PCR技术，但目前我们的研究需要准确定量的反应。为此，Q-PCR机的可用性是必不可少的。除了它对所追求的项目的科学相关性的贡献之外，这台设备对于学生和实验室其他人员的第一手培训非常重要。Q-PCR是目前在全国医院临床分析中广泛使用的技术。

Real time (RT) polymerase chain reaction (PCR), also known as quantitative PCR (Q-PCR), is a method that amplifies fragments of nucleic acids of known nucleotide sequence and is able to quantify the often very small amounts of this nucleic acid in the original sample. Several aspects of the research pursued in my laboratory (currently funded by NSERC) involve the characterization of the genes encoding proteins that are involved in the cell differentiation process (spermatogenesis) that leads to the formation of male sperm cells (spermatozoa). We are also very interested in the characterization of the proteins that interact with sperm immediately after fertilization of the egg and their gene expression. Therefore, much of our current work involves the analysis and characterization of genes from different vertebrate and invertebrate organisms and the timing and amount of their expression using Q-PCR. In addition to understanding the basic molecular mechanisms involved in spermatogenesis and early embryogenesis, this research has important implications for infertility. In our lab we have been using the PCR technique extensively in the past but the current needs of our research require the accurate quantification of the reaction. To this end, the availability of a Q-PCR machine is imperative. In addition to its contribution to the scientific relevance of the project pursued, this piece of equipment is very important for first-hand training of students and other personnel in the lab. Q-PCR is a widespread current technique used in clinical analysis at hospitals across the country.