Quantitative polymerase chain reaction system

定量聚合酶链式反应系统

• 批准号: 375619-2009
• 负责人: Ausió, Juan
• 金额: $ 2.88万
• 依托单位: University of Victoria
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412189
• 关键词: Quantitative; polymerase; chain; reaction; system

Real time (RT) polymerase chain reaction (PCR), also known as quantitative PCR (Q-PCR), is a method that amplifies fragments of nucleic acids of known nucleotide sequence and is able to quantify the often very small amounts of this nucleic acid in the original sample. Several aspects of the research pursued in my laboratory (currently funded by NSERC) involve the characterization of the genes encoding proteins that are involved in the cell differentiation process (spermatogenesis) that leads to the formation of male sperm cells (spermatozoa). We are also very interested in the characterization of the proteins that interact with sperm immediately after fertilization of the egg and their gene expression. Therefore, much of our current work involves the analysis and characterization of genes from different vertebrate and invertebrate organisms and the timing and amount of their expression using Q-PCR. In addition to understanding the basic molecular mechanisms involved in spermatogenesis and early embryogenesis, this research has important implications for infertility. In our lab we have been using the PCR technique extensively in the past but the current needs of our research require the accurate quantification of the reaction. To this end, the availability of a Q-PCR machine is imperative. In addition to its contribution to the scientific relevance of the project pursued, this piece of equipment is very important for first-hand training of students and other personnel in the lab. Q-PCR is a widespread current technique used in clinical analysis at hospitals across the country.

实时(RT)聚合酶链式反应(PCR)，也称为定量聚合酶链式反应(Q-PCR)，是一种扩增已知核苷酸序列的核酸片段的方法，能够对原始样本中通常非常少量的这种核酸进行定量。我的实验室(目前由NSERC资助)进行的研究的几个方面涉及到编码蛋白质的基因的特征，这些蛋白质参与了导致男性精子细胞(精子)形成的细胞分化过程(精子发生)。我们还对卵子受精后立即与精子相互作用的蛋白质及其基因表达的特征非常感兴趣。因此，我们目前的大部分工作包括分析和表征不同脊椎动物和无脊椎动物的基因，以及使用Q-PCR来确定它们的表达时间和数量。除了了解精子发生和早期胚胎发生的基本分子机制外，这项研究还对不育症具有重要意义。在我们的实验室里，我们过去一直在广泛地使用PCR技术，但目前我们研究的需要要求对反应进行准确的定量。为此，Q-PCR机的可用性势在必行。除了对所追求的项目的科学相关性做出贡献外，这台设备对于实验室中学生和其他人员的第一手培训非常重要。Q-PCR是目前在全国医院临床分析中广泛使用的一种技术。