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Accelerated evolution of enzyme activity to determine functional and structural tolerance

Accelerated evolution of enzyme activity to determine functional and structural tolerance
酶活性的加速进化以确定功能和结构耐受性
批准号:
227853-2010
负责人:
Pelletier, Joelle
金额:
$3.64万
依托单位:
依托单位国家:
加拿大
项目类别:
Discovery Grants Program - Individual
财政年份:
2011
资助国家:
加拿大
项目状态:
已结题
起止时间:
2011-01-01 至 2012-12-31

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英文摘要
The industrial application of enzymes is gaining in importance world-wide, as a result of their high catalytic power and the potential for modification by enzyme engineering. Our goal is to increase the scope and efficiency of enzyme engineering, by determining the tolerance of specific enzymes to intensive, targeted mutational efforts. Different enzymes appear to show different tolerance to active-site mutations. Here, we propose to study two different, unrelated enzyme systems. Following extensive modification using powerful molecular biological methodologies, we will characterize the functional variants to establish their tolerance to intensive, targeted mutations.
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    RGPIN-2018-04686
  • 项目类别:
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  • 批准号:
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Engineering enzymes in all their dimensions: from inhibition to biotransformation
  • 批准号:
    RGPIN-2018-04686
  • 项目类别:
    Discovery Grants Program - Individual
  • 资助金额:
    $6.85万
  • 财政年份:
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  • 批准号:
    CRC-2020-00171
  • 项目类别:
    Canada Research Chairs
  • 资助金额:
    $14.57万
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  • 负责人:
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