Accelerated evolution of enzyme activity to determine functional and structural tolerance

酶活性的加速进化以确定功能和结构耐受性

• 批准号: 227853-2010
• 负责人: Pelletier, Joelle
• 金额: $ 3.64万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=546845
• 关键词: Accelerated; evolution; enzyme; activity; determine

The industrial application of enzymes is gaining in importance world-wide, as a result of their high catalytic power and the potential for modification by enzyme engineering. Our goal is to increase the scope and efficiency of enzyme engineering, by determining the tolerance of specific enzymes to intensive, targeted mutational efforts. Different enzymes appear to show different tolerance to active-site mutations. Here, we propose to study two different, unrelated enzyme systems. Following extensive modification using powerful molecular biological methodologies, we will characterize the functional variants to establish their tolerance to intensive, targeted mutations.

由于酶的高催化能力和酶工程修饰的潜力，酶的工业应用在世界范围内越来越重要。我们的目标是通过确定特定酶对密集的靶向突变的耐受性来增加酶工程的范围和效率。不同的酶对活性位点突变表现出不同的耐受性。在这里，我们建议研究两个不同的，不相关的酶系统。在使用强大的分子生物学方法进行广泛修饰后，我们将对功能变异进行表征，以确定它们对密集靶向突变的耐受性。