High-throughput homogenizer for analysis of gene expression and metabolic profiles

本申请申请的资金用于购买配备crylyys冷却系统的高通量Precellys 24组织均质机。这是唯一的市售高通量组织均质机与冷却选项，这是必要的温度敏感样品的处理。分解组织（均质化）以释放细胞化合物通常是生物化学或遗传分析的第一步，这一过程可能是最耗时的过程之一。使用组织匀浆的标准技术，学生和研究人员可能要在匀浆机前花费数周时间，但使用新的高通量技术，如Precellys 24组织匀浆机，处理样品的时间可以减少5到10倍。能够快速处理数百或数千个组织样本非常重要，这样数据才能以最省时的方式发表。这种高通量均质机将直接支持博士的研究计划。舒尔特总理和理查兹。他们的研究项目试图确定动物（主要是鱼类）如何应对环境挑战。从人类对环境的影响日益增加的角度来看，这一研究目标具有特别的相关性，因为生物体正在经历前所未有的环境变化，这些变化正在考验它们的容忍极限。了解设定物种承受极限的因素是预测哪些动物因全球变化而面临最大风险的重要组成部分。

The funding requested in this grant application is to support the purchase of a high-throughput Precellys 24 tissue homogenizer with the Cryolys cooling system. This is the only commercially available high-throughput tissue homogenizer with a cooling option, which is necessary for the processing of temperature sensitive samples. Breaking apart tissues (homogenization) to release cellular compounds is the often the first step in any biochemical of genetic analysis of organisms and this process can be one of the most time consuming. Using standard technology for tissue homogenization, students and researchers can spend weeks in front of a homogenizer, but with new, high-throughput technology, such as the Precellys 24 Tissue homogenizer, time spent processing samples can be reduced by up to 5 or 10 fold. Being able to process hundreds or thousands of tissue samples quickly is important so that data can reach publication in the most time efficient manner possible. This high-throughput homogenizer will directly support the research programs of Drs. PM Schulte and JG Richards. Combined, their research programs attempt to determine how animals, mostly fish, respond to environmental challenge. This research goal is of particular relevance when viewed from the perspective of increasing human impacts on the environment, because organisms are now experiencing unprecedented environmental changes that are testing their tolerance limits. Understanding the factors that set a species' tolerance limits is an important component of predicting which animals are most at risk due to global change.