Improving Capacity to Increase Throughput and Minimize Cross-Contamination During Sample Processing Utilizing a Bead Mill Homogenizer

项目摘要/摘要 该项目涉及购买全向珠磨机均质机和相关的 增加生产量和减少样品加工过程中的交叉污染的供应品。在一次 需要对大量样本进行快速分析的已知或疑似疾病爆发， 在诊断测试中，样品制备常常是一个限速步骤。饲料的交叉污染 和其他动物产品可以发生在制造、搬运、运输、储存或在 并可能导致无意和潜在有害的接触过量的 维生素、矿物质、人类或兽药、杀虫剂或其他潜在有害物质 物质。偶尔，诊断样本可能会发生意外的交叉污染 采集样品或在实验室分析的准备和处理过程中。我们的 实验室目前充当VET-LIRN网络实验室，以诊断、表征和报告 新出现的和地方性兽医疾病，包括动物中毒和食品或饲料污染 事件。此次采购将使我们能够同时处理3到12个样品，而目前的 一次只能处理一个样品的均质装置。此高通量样本 处理能力将大大减少样品准备时间和所涉及的人员劳动。 最后，这种珠磨均质机为每个样品使用一个单独的样品管，避免了 使用相同的匀化刀片时可能发生的潜在交叉污染 样品与我们目前的设备相同。

PROJECT SUMMARY/ABSTRACT This project involves the purchase of an Omni Bead Ruptor bead mill homogenizer and associated supplies to increase throughput and minimize cross-contamination during sample processing. During a known or suspected disease outbreak that requires rapid analysis of large numbers of specimens, sample preparation is frequently a rate-limiting step in diagnostic testing. Cross-contamination of feed and other animal products can occur during manufacturing, handling, transport, storage, or at the point of consumption and may result in unintentional and potentially harmful exposures to excessive levels of vitamins, minerals, human or veterinary medications, pesticides, or other potentially harmful substances. Occasionally, accidental cross-contamination of diagnostic specimens may occur during either sample collection or in the course of preparation and processing for laboratory analysis. Our laboratory currently serves as a Vet-LIRN network laboratory to diagnose, characterize and report emerging and endemic veterinary diseases, including animal poisonings and food or feed contamination events. This purchase will enable us to process 3 to 12 samples simultaneously, compared to our current homogenization device that can only process one sample at a time. This high-throughput sample processing capacity will greatly reduce sample preparation time and the personnel labor involved. Finally, this bead mill homogenizer utilizes an individual sample tubes for each sample, avoiding the potential cross-contamination that may occur when the same homogenization blade is used for each sample as with our current device.