Fluorescence microplate reader for solution- and cell-based assays

用于溶液和细胞分析的荧光酶标仪

• 批准号: 422043-2012
• 负责人: LeMoual, Hervé
• 金额: $ 1.85万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495713
• 关键词: Fluorescence; microplate; reader; solution; cell

The Department of Microbiology and Immunology, at McGill University, maintains a common equipment core resource. This application is submitted for the purchase of a fluorescence-microplate reader, which is currently unavailable. Five NSERC-supported researchers or Canada Research Chairs currently supervising 22 graduate students and postdoctoral fellows are part of this application. Taking advantage of the high sensitivity of fluorescence and the wide range of fluorescent labels available today, researchers of this Department will develop various solution- and cell-based assays. These new assays comprise fluorescence resonance energy transfer assays, enzymatic assays and whole-cell binding assays. By switching from existing radioisotope-based assays to fluorescence-based assays, researchers will be able to drastically reduce the amount of radioactive waste generated and lower the cost associated with waste disposal. In turn, this will make our research environment safer. In addition, the requested microplate reader will be pivotal to the development of high-throughput screening of small-molecule libraries to identify lead compounds. Upon optimization, these lead compounds may become tomorrow's medicines.

麦吉尔大学的微生物学和免疫学系拥有一个共同的核心设备资源。本申请是为了购买荧光酶标仪而提交的，目前没有。五名NSERC支持的研究人员或加拿大研究主席目前监督22名研究生和博士后研究员是本申请的一部分。利用荧光的高灵敏度和广泛的荧光标记的优势，本部门的研究人员将开发各种基于溶液和细胞的检测。这些新的检测包括荧光共振能量转移检测、酶检测和全细胞结合检测。通过从现有的基于放射性同位素的检测转换为基于荧光的检测，研究人员将能够大幅减少放射性废物的产生量，并降低与废物处理相关的成本。反过来，这将使我们的研究环境更安全。此外，所需的酶标仪对于开发高通量筛选小分子库以识别先导化合物至关重要。经过优化，这些先导化合物可能成为明天的药物。