Multimode Fluorescence Microplate Reader

描述（由申请人提供）：巴尔的摩的马里兰州大学（UMB）的NIH资助的主要研究者（PI）对最先进的多模式酶标仪有科学需求，以支持和增强其NIH资助的研究。该仪器将位于马里兰州大学Marlene和Stewart Greenebaum癌症中心（UMCCC）高通量筛查共享服务（HTS SS）并由其管理，并将提供给癌症中心的成员以及整个UMB的PI。酶标仪将取代HTS SS中目前使用约10年的设备，并在6至3456孔微孔板中自动收集吸光度、荧光强度（FI）、荧光偏振（FP）、时间分辨荧光（TR）、TR-荧光共振能量转移（TR-FRET）、发光和AlphaScreen功能。HTS SS中可用的现有酶标仪不具有自动加载能力，限于384孔格式或更少，在FP模式下缺乏灵敏度，不能进行基于镧系元素的TR-FRET测定或AlphaScreen测定，其不具有用于双荧光素酶测定或快速酶动力学测量的机载流体注射器，并且缺乏UMB研究者所需的总体速度和灵敏度。这种新型多模式酶标仪将是HTS SS提供的服务的核心，对于支持国家癌症研究所（NCI）指定的UMCCC的基础科学和药物发现计划以及由NIH资助的整个UMB的PI研究至关重要。

DESCRIPTION (provided by applicant): NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art multimode microplate reader to support and enhance their NIH funded research. This instrument will be located in and managed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) High Throughput Screening Shared Service (HTS SS) and will be available for members of the Cancer Center as well as for PIs throughout UMB. The microplate reader will replace the ~10 year old equipment currently in the HTS SS, and provide automated collection of absorbance, fluorescence intensity (FI), fluorescence polarization (FP), time resolve fluorescence (TR), TR-Fluorescence Resonance Energy Transfer (TR-FRET), luminescence, and AlphaScreen capabilities in 6 to 3456-well microplates. The existing microplate reader available in the HTS SS does not have auto-loading capabilities, is limited to 384- well format or less, lacks sensitivity in the FP mode, is not capable of lanthanide based TR-FRET assays or AlphaScreen assays, it does not have on-board fluid injectors for dual luciferase assays or rapid enzyme kinetic measurements, and lacks the overall speed and sensitivity needed by UMB investigators. This new multimode microplate reader will be central to the services provided by our HTS SS and will be critical to support basic science and drug discovery programs in the National Cancer Institute (NCI) designated UMGCC as well as for research of PI's throughout UMB who are funded by the NIH.