Multimode Fluorescence Microplate Reader

描述（由申请人提供）：美国国立卫生研究院资助的马里兰大学巴尔的摩分校（UMB）的首席研究员（pi）对最先进的多模式微孔板阅读器有科学需求，以支持和加强他们的美国国立卫生研究院资助的研究。该仪器将位于马里兰大学马琳和斯图尔特格林鲍姆癌症中心（UMGCC）高通量筛选共享服务（HTS SS），并由其管理，将为癌症中心的成员以及UMB的pi提供服务。该微孔板读取器将取代目前HTS SS中已有10年历史的设备，并在6至3456孔微孔板中提供吸光度、荧光强度（FI）、荧光偏振（FP）、时间分辨荧光（TR）、TR-荧光共振能量转移（TR- fret）、发光和alphasgreen功能的自动收集。HTS SS中现有的微孔板读取器不具有自动加载功能，仅限于384孔格式或更少，在FP模式下缺乏灵敏度，不能进行基于镧系元素的TR-FRET测定或AlphaScreen测定，它没有用于双荧光素酶测定或快速酶动力学测量的车载流体注射器，缺乏UMB研究者所需的总体速度和灵敏度。这种新的多模式微孔板阅读器将是我们HTS SS提供服务的核心，对于支持国家癌症研究所（NCI）指定的UMGCC的基础科学和药物发现项目以及由NIH资助的UMB PI的研究至关重要。

DESCRIPTION (provided by applicant): NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art multimode microplate reader to support and enhance their NIH funded research. This instrument will be located in and managed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) High Throughput Screening Shared Service (HTS SS) and will be available for members of the Cancer Center as well as for PIs throughout UMB. The microplate reader will replace the ~10 year old equipment currently in the HTS SS, and provide automated collection of absorbance, fluorescence intensity (FI), fluorescence polarization (FP), time resolve fluorescence (TR), TR-Fluorescence Resonance Energy Transfer (TR-FRET), luminescence, and AlphaScreen capabilities in 6 to 3456-well microplates. The existing microplate reader available in the HTS SS does not have auto-loading capabilities, is limited to 384- well format or less, lacks sensitivity in the FP mode, is not capable of lanthanide based TR-FRET assays or AlphaScreen assays, it does not have on-board fluid injectors for dual luciferase assays or rapid enzyme kinetic measurements, and lacks the overall speed and sensitivity needed by UMB investigators. This new multimode microplate reader will be central to the services provided by our HTS SS and will be critical to support basic science and drug discovery programs in the National Cancer Institute (NCI) designated UMGCC as well as for research of PI's throughout UMB who are funded by the NIH.