Multimode Fluorescence Microplate Reader

多模式荧光酶标仪

• 批准号: 8052483
• 负责人: David Joseph Weber
• 金额: $ 16.44万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052483
• 关键词: 10 year old; Baltimore; Basic Science; Biological Assay; Cancer Center; Collection; Enzyme Kinetics; Equipment; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Funding; Lanthanoid Series Elements; Liquid substance; Luciferases; Maryland; Measurement; National Cancer Institute; Principal Investigator; Reader; Research; Research Personnel; Services; Speed; Time; United States National Institutes of Health; Universities; base; drug discovery; high throughput screening; instrument; luminescence; member; programs

DESCRIPTION (provided by applicant): NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art multimode microplate reader to support and enhance their NIH funded research. This instrument will be located in and managed by the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) High Throughput Screening Shared Service (HTS SS) and will be available for members of the Cancer Center as well as for PIs throughout UMB. The microplate reader will replace the ~10 year old equipment currently in the HTS SS, and provide automated collection of absorbance, fluorescence intensity (FI), fluorescence polarization (FP), time resolve fluorescence (TR), TR-Fluorescence Resonance Energy Transfer (TR-FRET), luminescence, and AlphaScreen capabilities in 6 to 3456-well microplates. The existing microplate reader available in the HTS SS does not have auto-loading capabilities, is limited to 384- well format or less, lacks sensitivity in the FP mode, is not capable of lanthanide based TR-FRET assays or AlphaScreen assays, it does not have on-board fluid injectors for dual luciferase assays or rapid enzyme kinetic measurements, and lacks the overall speed and sensitivity needed by UMB investigators. This new multimode microplate reader will be central to the services provided by our HTS SS and will be critical to support basic science and drug discovery programs in the National Cancer Institute (NCI) designated UMGCC as well as for research of PI's throughout UMB who are funded by the NIH.

描述(由申请人提供)：美国国立卫生研究院资助的巴尔的摩马里兰大学(UMB)首席研究员(PI)需要一种最先进的多模式微孔板阅读器来支持和加强他们由NIH资助的研究。该仪器将位于马里兰大学马里兰分校和斯图尔特·格林鲍姆癌症中心(UMGCC)高通量筛查共享服务(HTS SS)内并由其管理，癌症中心成员以及整个UMB的PI都可以使用该仪器。微板阅读器将取代HTS SS中目前已有约10年历史的设备，并在6至3456孔微板中提供吸光度、荧光强度(FI)、荧光偏振(FP)、时间分辨荧光(TR)、TR荧光共振能量转移(TR-FRET)、发光和AlphaScreen功能的自动收集。HTS SS中现有的微孔板阅读器没有自动加载能力，限制在384孔或更小的格式，在FP模式下缺乏灵敏度，不能进行基于稀土的TRRET或AlphaScreen分析，它没有用于双荧光素酶分析或快速酶动力学测量的板载流体注射器，并且缺乏UMB调查人员所需的整体速度和灵敏度。这种新的多模式微板读取器将是我们HTS SS提供的服务的核心，对于支持国家癌症研究所(NCI)指定的UMGCC的基础科学和药物发现计划以及由NIH资助的UMB各地的PI研究至关重要。