In situ quantification of gold nanoparticles using the total reflection X-ray fluorescence

使用全反射 X 射线荧光原位定量金纳米颗粒

• 批准号: 422685-2012
• 负责人: PejovicMilic, Ana
• 金额: $ 9.27万
• 依托单位: Ryerson University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495836
• 关键词: situ; quantification; gold; nanoparticles; using

This proposal is for the purpose of purchasing a total reflection X-ray fluorescence (TXRF) spectrometer, and associated equipment, for the purpose of the in situ low-level quantification of gold, in the form of gold nanoparticle, in cell cultures and tissues, a fundamental, and currently limiting requirement for the advancement of the study of nanoparticle aided therapies. The application of ultrasound-microbubbles in combination with gold nanoparticles is being investigated to improve therapeutic outcome of radiotherapy and laser-mediated thermal therapy of cancerous tissues. Although the general physical mechanism of the action of gold nanoparticles in aiding such therapies is largely known, the optimal method of therapeutic delivery, and an understanding of gold nanoparticle uptake into cells, is still missing. This requires that an accurate in situ measure of gold delivered to cancerous cells be known. Gold quantification is a challenge in such a case as most wet chemical methods require total destruction of the sample, and do not generally present limits of detection which are sufficient to detect and quantify the very low levels of gold expected in cells after treatment with gold nanoparticles. TXRF offers very low limits of detection (at ppb levels), and being based on X-ray fluorescence allows for complete in situ quantification of gold in thin layer samples, including, mono- and multi-layer cell cultures. A similar, but complementary, area of potential application of TXRF would be in the assessment of other trace elements in human tissues, such as in teeth or bone, or in environmental, archeological and art samples in the future.

该建议是为了购买总反射X射线荧光（TXRF）光谱仪和相关的设备，目的是以金纳米粒子的形式，在细胞培养物和组织中以金纳米粒子的形式进行原位的低水平量化，这是一种基本的，目前是对Nanaport seride thered thersed thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered thered ther thered thered thered thered thered的限制要求。正在研究超声 - 微泡与金纳米颗粒结合使用，以改善放射疗法的治疗结果和激光介导的癌组织的热治疗。尽管金纳米颗粒在辅助这种疗法中的作用的一般物理机制在很大程度上是已知的，最佳的治疗递送方法以及对金纳米颗粒摄取到细胞的理解仍然缺失。这就要求已知准确的原位将传递给癌细胞的黄金的原位度量。在这种情况下，黄金定量是一个挑战，因为大多数湿化学方法需要对样品进行全面破坏，并且通常不存在检测限制，这足以检测和量化用金纳米颗粒处理后的细胞中预期的非常低的黄金水平。 TXRF提供了非常较低的检测极限（在PPB水平下），并且基于X射线荧光允许在薄层样品中对金的原位定量，包括单层和多层细胞培养物。 TXRF潜在应用的类似但互补的领域将是评估人类组织中其他痕量元素的评估，例如牙齿或骨骼，或将来的环境，考古和艺术样本。