Precision Microinjection system for cell fate mapping in embryogenesis

在这个建议中，我要求一个数字控制的显微注射系统，用于标记海绵胚胎中的细胞，并跟踪它们在成人体内的命运。所要求的设备将允许学生使用荧光标记研究单个细胞的特定活动，并使用荧光成像技术和3D重建软件跟踪它们在胚胎和幼年动物中的位置。需要一种高度控制的定位细胞的方法以及一种精确的应用染料的方法。由于手动操作器的不精确和粗糙运动，在没有这种设备的情况下进行这种操作的尝试失败了。新仪器将显著提高我的研究生和同事的易用性，从而提高用户时间效率和培训。

In this proposal I request a digitally controlled microinjection system for labeling cells in embryos of sponges and following their fate in the adult body. The requested equipment will allow students to study the specific activities of individual cells using fluorescent markers and follow their position in the embryos and juvenile animals with fluorescent imaging techniques and 3D reconstruction software. A highly controlled method of locating the cells is needed as well as a precise method of applying the dye. Attempts to do this without this equipment have failed due to the imprecision and rough movement of manual manipulators. The new instrument will significantly improve ease of use by my graduate students and associates thereby increasing both user-time efficiency and training.