Oxygen microsensor for fluorescent detection of oxygen in sponges

在这项提案中，我请求为一个氧气微传感器系统提供资金，以便同时记录环境水和海绵过滤水中的氧气水平。所要求的设备将使学生能够研究海绵在不同环境条件下（低流量和高流量）的代谢以及对药理学和基因敲除治疗的反应。需要一种准确、稳定和稳健的方法来量化海绵的氧气使用，同时需要能够从控制和实验位置进行记录。借用仪器的工作表明，这些数据对于计算海绵功能的能量学是多么有价值，但也突出了早期技术在探针尖端漂移和脆弱性方面的困难。拟议的仪器使用了新的非常稳定的技术，已被证明是非常有效的。该仪器是3名研究生的具体实验所急需的，他们的结果将被研究小组的另外两名成员用于建模。使用这种微传感器的能力将提高我所有小组成员的生产力。

In this proposal I request funds for an oxygen microsensor system for simultaneous recording of oxygen levels in ambient water and water filtered by sponges. The requested equipment will allow students to study the metabolism of sponges under different ambient conditions (low and high flow) and in response to pharmacological and gene knockdown treatments. An accurate, stable and robust method of quantifying oxygen use by the sponge is needed, as is the ability to record from control and experimental locations simultaneously. Work with borrowed instruments has shown how valuable this data is for calculating energetics of sponge function, but also highlighted difficulties with earlier technologies in terms of drift and fragility of probe tips. The proposed instrument uses new very stable technology that has proven very effective. This instrument is urgently needed for specific experiments of 3 graduate students and their results will be used by two other members of the research group for modeling. The ability to use this microsensor will enhance productivity for all my group members.