Oxygen microsensor for fluorescent detection of oxygen in sponges

在本提案中，我请求资助一个氧气微传感器系统，用于同时记录环境水和海绵过滤水中的氧气水平。所要求的设备将使学生能够研究海绵在不同环境条件下的代谢（低流量和高流量）以及对药物和基因敲除治疗的反应。需要一种准确、稳定和可靠的方法来定量海绵的氧气使用，同时需要从控制和实验位置进行记录的能力。利用借来的仪器进行的工作表明，这些数据对于计算海绵功能的能量学是多么有价值，但也突出了早期技术在漂移和探针尖端脆弱性方面的困难。拟议的仪器使用了新的非常稳定的技术，已被证明非常有效。3名研究生的具体实验急需该仪器，其结果将由课题组的另外两名成员进行建模。使用这种微传感器的能力将提高我所有小组成员的生产力。

In this proposal I request funds for an oxygen microsensor system for simultaneous recording of oxygen levels in ambient water and water filtered by sponges. The requested equipment will allow students to study the metabolism of sponges under different ambient conditions (low and high flow) and in response to pharmacological and gene knockdown treatments. An accurate, stable and robust method of quantifying oxygen use by the sponge is needed, as is the ability to record from control and experimental locations simultaneously. Work with borrowed instruments has shown how valuable this data is for calculating energetics of sponge function, but also highlighted difficulties with earlier technologies in terms of drift and fragility of probe tips. The proposed instrument uses new very stable technology that has proven very effective. This instrument is urgently needed for specific experiments of 3 graduate students and their results will be used by two other members of the research group for modeling. The ability to use this microsensor will enhance productivity for all my group members.