Characterising novel signalling molecules important for sperm fertilization

表征对精子受精重要的新型信号分子

• 批准号: 105823-2007
• 负责人: Buhr, Mary
• 金额: $ 2.41万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=504164
• 关键词: Characterising; novel; signalling; molecules; important

The molecules in mammalian sperm must reorganise just prior to meeting the egg, in order to fertilise. This reorganisation is started by signals that the sperm receives which cause the sperm to change its membrane and change its internal reactions. The exact nature of the signal(s) is unknown, making it difficult to either protect fertilising ability in males with poor fertility or to preserve sperm for artificial insemination. One molecule called Na/K-ATPase is well known as an enzyme that moves ions across the membranes of many cell types, including sperm. We have recently proven that Na/K-ATPase is a signalling molecule like a hormone receptor, and its hormone has recently been identified, in muscle cells, as the steroid ouabain. We propose to determine how ouabain's signal is received and acted upon by sperm; what its role is in membrane changes that preceded fertilisation, and how the molecules surrounding it in its immediate membrane environment can be manipulated to better protect and promote fertilisation.

哺乳动物精子中的分子必须在与卵子相遇之前重组，才能受精。这种重组是由精子接收到的信号开始的，这些信号导致精子改变其膜并改变其内部反应。信号的确切性质尚不清楚，这使得保护生育力差的男性的受精能力或保存精子用于人工授精变得困难。一种叫做Na/ k - atp酶的分子是一种众所周知的酶，它可以使离子穿过许多细胞类型的膜，包括精子。我们最近已经证明Na/ k - atp酶是一种类似激素受体的信号分子，它的激素最近在肌肉细胞中被鉴定为类固醇瓦巴因。我们打算确定精子是如何接收和作用瓦巴因的信号的；它在受精前的膜变化中起什么作用，以及在其直接膜环境中如何操纵其周围的分子以更好地保护和促进受精。