Multi-user fluorescence microscope for live cell imaging

用于活细胞成像的多用户荧光显微镜

• 批准号: 440381-2013
• 负责人: Duncan, Roy
• 金额: $ 10.89万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2012
• 资助国家: 加拿大
• 起止时间: 2012-01-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=505653
• 关键词: Multi; user; fluorescence; microscope; live

This proposal is to acquire an upgrade to an existing microscope that will allow 9 researchers and their trainees to monitor the changes in cellular activity in real-time. Powerful new techniques now allow researchers to attach fluorescent tags to individual proteins. Researchers can now follow individual proteins as they move through cells and interact with other proteins and cellular membranes using a fluorescence microscope with live cell imaging capability. This technology can be applied to understand how proteins interact with each other or respond to different treatments, and how these proteins work alone or together with other proteins to regulate or alter the normal function of cells. Studies performed with this live cell imaging system will provide important insights into normal cellular function and how cells respond to various stresses.

这项提议是对现有显微镜进行升级，使9名研究人员和他们的受训人员能够实时监测细胞活动的变化。强大的新技术现在允许研究人员在单个蛋白质上贴上荧光标签。研究人员现在可以使用具有活细胞成像能力的荧光显微镜来跟踪单个蛋白质在细胞中移动，并与其他蛋白质和细胞膜相互作用。这项技术可以用来了解蛋白质如何相互作用或对不同的治疗做出反应，以及这些蛋白质如何单独或与其他蛋白质一起调节或改变细胞的正常功能。使用这种活细胞成像系统进行的研究将为了解正常的细胞功能和细胞如何应对各种压力提供重要的见解。