Benchtop ultracentrifuge for molecular cell biology

用于分子细胞生物学的台式超速离心机

• 批准号: 422637-2012
• 负责人: Duncan, Roy
• 金额: $ 7.73万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495832
• 关键词: Benchtop; ultracentrifuge; molecular; cell; biology

Understanding the complex processes that regulate how cells function frequently requires being able to separate the various constituents that comprise a cell. For example, the ability to separate the membrane fraction of cells from the soluble fraction, or various membrane compartments (e.g. nucleus, mitochondria, endoplasmic reticulum) from each other allows researchers to investigate how these structures are changed as a result of metabolic processes. Researchers also frequently need to purify individual cellular or viral protein complexes (e.g. ribosomes, virus particles) or even individual proteins. The best way to achieve these goals is using ultracentrifugation, where disrupted cells are centrifuged at high forces of gravity allowing the separation of cell constituents based on their relative size or density. The requested equipment is the newest version of a benchtop ultracentrifuge that will replace our existing centrifuge that is over 20 years old and no longer dependable. This equipment upgrade will provide a large group of researchers and their trainees with the necessary equipment needed to pursue a diverse range of investigations aimed at understanding how cells function.

要了解调控细胞功能的复杂过程，需要能够分离组成细胞的各种成分。例如，将细胞膜部分与可溶性部分或不同的膜隔间(如细胞核、线粒体、内质网)相互分离的能力使研究人员能够研究这些结构是如何随着代谢过程而改变的。研究人员还经常需要提纯单个细胞或病毒蛋白质复合体(如核糖体、病毒颗粒)，甚至单个蛋白质。实现这些目标的最好方法是使用超速离心法，即在高重力下分离破碎的细胞，允许根据细胞的相对大小或密度分离细胞成分。所要求的设备是最新版本的台式超速离心机，它将取代我们现有的离心机，该离心机已有20多年的历史，不再可靠。这次设备升级将为一大批研究人员及其受训人员提供必要的设备，以进行旨在了解细胞如何工作的各种调查。