Benchtop ultracentrifuge for molecular cell biology

理解调节细胞功能的复杂过程需要能够分离组成细胞的各种成分。例如，将细胞的膜部分与可溶性部分分离的能力，或将各种膜区室（如细胞核、线粒体、内质网）相互分离的能力，使研究人员能够研究这些结构是如何因代谢过程而改变的。研究人员还经常需要纯化单个细胞或病毒蛋白复合物（如核糖体、病毒颗粒），甚至单个蛋白质。实现这些目标的最佳方法是使用超离心，在这种方法中，被破坏的细胞在高重力下离心，从而根据细胞的相对大小或密度分离细胞成分。所要求的设备是台式超离心机的最新版本，将取代我们现有的超过20年的离心机，不再可靠。这一设备升级将为一大群研究人员和他们的学员提供必要的设备，以进行旨在了解细胞功能的各种调查。

Understanding the complex processes that regulate how cells function frequently requires being able to separate the various constituents that comprise a cell. For example, the ability to separate the membrane fraction of cells from the soluble fraction, or various membrane compartments (e.g. nucleus, mitochondria, endoplasmic reticulum) from each other allows researchers to investigate how these structures are changed as a result of metabolic processes. Researchers also frequently need to purify individual cellular or viral protein complexes (e.g. ribosomes, virus particles) or even individual proteins. The best way to achieve these goals is using ultracentrifugation, where disrupted cells are centrifuged at high forces of gravity allowing the separation of cell constituents based on their relative size or density. The requested equipment is the newest version of a benchtop ultracentrifuge that will replace our existing centrifuge that is over 20 years old and no longer dependable. This equipment upgrade will provide a large group of researchers and their trainees with the necessary equipment needed to pursue a diverse range of investigations aimed at understanding how cells function.