Real Time Polymerase Chain Reaction Machine

这个RTI申请的主要申请人是David Wright博士。大卫·戴克，杰里米·辛普森和阿曼达·赖特作为共同申请人。D.Wright博士的实验室研究运动和饮食对脂肪组织、肝功能和新陈代谢的调节。Wright博士的基础研究项目的主要目标是阐明胰高血糖素在控制肝源性激素（称为肝因子）分泌中的作用和潜在机制，并反过来研究这些变化如何影响全身碳水化合物和脂质代谢。该项目由NSERC发现基金资助。肝因子基因表达的分析是这些实验的中心结果测量。D.Wright博士的研究与Dyck博士正在进行的nserc资助的研究相吻合，Dyck博士的研究重点是控制骨骼肌中碳水化合物和脂质的摄取、氧化和储存的基本机制，以及这些复杂的过程是如何由脂肪组织衍生的信号因子（称为脂肪因子）调节的。Dyck博士和D.Wright博士的机械代谢研究项目补充了Jeremy Simpson博士的NSERC资助项目，该项目研究骨骼肌中收缩蛋白的翻译后修饰，以及这些蛋白是如何随着年龄、饮食和运动而改变的。最后，阿曼达·赖特博士的NSERC资助的研究旨在更好地了解脂质分子结构和物理特性在调节营养功能的吸收前消化事件中的基本作用。总的来说，本提案申请人的研究项目需要广泛分析基因表达的变化，因为它涉及多个系统，包括骨骼肌，脂肪组织，肝脏和肠道功能和代谢。因此，申请人正在申请资金，以购买一台新的RT-PCR机进行这些测量。从申请人实验室正在进行的研究中产生的数据将增加我们对各种营养和生理压力下控制骨骼肌，脂肪组织，肝脏和肠道功能和代谢的机制的基本理解。

The principal applicant for this RTI application is Dr. David Wright with Drs. David Dyck, Jeremy Simpson and Amanda Wright serving as co-applicants. Dr. D.Wright's laboratory examines the mediators of exercise and diet on the regulation of adipose tissue and liver function and metabolism. The main objective of Dr. Wright's basic research program, which is funded through an NSERC Discovery Grant, is to elucidate the role and underlying mechanisms of the hormone glucagon in controlling the secretion of liver derived hormones called hepatokines, and, in turn, examining how these alterations impact whole body carbohydrate and lipid metabolism. The analysis of hepatokine gene expression is a central outcome measure of these experiments. Dr. D.Wright's research dovetails with the on-going NSERC-funded investigations in Dr. Dyck's research laboratory which focuses on the basic mechanisms controlling the uptake, oxidation and storage of carbohydrate and lipid in skeletal muscle and how these intricate processes are regulated by adipose tissue derived signaling factors called adipokines. The mechanistic, metabolic research programs of Drs Dyck and D.Wright complement Dr. Jeremy Simpson's NSERC funded program that examines the post-translational modifications of contractile proteins in skeletal muscle and how these are altered by aging, diet and exercise. Finally Dr. Amanda Wright's NSERC funded research seeks to better understand the fundamental role of lipid molecular structure and physical properties on pre-absorptive digestive events that regulate nutritional functionality. Collectively, the research programs of the applicants of this proposal require the extensive analysis of changes in gene expression as it relates to multiple systems, including skeletal muscle, adipose tissue, liver and gut function and metabolism. Thus, the applicants are requesting funding to purchase a new RT-PCR machine for these measurements. The data generated from the ongoing studies in the applicants laboratories will increase our basic understanding of the mechanisms controlling skeletal muscle, adipose tissue, liver and gut function and metabolism under a variety of nutritional and physiological stresses.