Environmental enclosure for the determination of fuel metabolism across a range of temperatures

用于确定一定温度范围内燃料代谢的环境外壳

• 批准号: RTI-2020-00023
• 负责人: Wright, David
• 金额: $ 10.91万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2019
• 资助国家: 加拿大
• 起止时间: 2019-01-01 至 2020-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=693684
• 关键词: Environmental; enclosure; determination; fuel; metabolism

This is a request for an Environment Enclosure to be used with our metabolic caging system for small rodents that will allow variable temperature control within the metabolic caging. This infrastructure is urgently needed as it will allow us to measure energy expenditure and whole-body substrate oxidation across a range of temperatures, capabilities that we do not currently possess. This need has become critical given the expansion of our NSERC funded research programs into the study of fuel metabolism in the cold and the increasingly recognized importance of housing rodents under thermal neutral conditions; indeed, a number of our recent papers and grant applications, have been criticized for their failure to measure energy expenditure under thermal neutral conditions. In order for us to remain internationally competitive and to continue to provide the highest standard of training to highly qualified personnel (HQP), it is essential for our research programs to be able to measure energy expenditure and fuel oxidation across a range of environmental temperatures. While other universities in the region have metabolic cages with the capacity to regulate temperature the transfer of animals between institutions is logistically challenging and can cause stress to the animals, which can confound experimental results. Moreover, experiments typically involve monitoring animals for several days at a time, which means there will be significant demands on the system which further limits the feasibility of accessing the required infrastructure at other institutions. Given these points, obtaining the requested infrastructure in-house is the only feasible way to measure energy expenditure across various temperatures.***The research programs of primary applicants are integrative in nature, where HQP are encouraged to consider all factors influencing biological systems. Measuring energy expenditure provides critical information regarding the response to various perturbations. Indeed, obtaining the requested infrastructure will allow us to consider alterations in energy expenditure and fuel utilization in the cold. These same endpoints will also be measured in experimental rodents housed at thermal neutrality, a condition which is increasingly being recognized as superior to room temperature housing for translatability to human physiology. Over 40 HQP from across 6 research laboratories will immediately and directly benefit from the acquisition of the requested equipment. This group includes 17 MSc, 14 PhD, 13 undergraduates and 4 post-doctoral fellows, a large and diverse group of trainees with strong representation from multiple equity seeking groups. Of note greater than 50% of the HQP in the laboratories of the anticipated users are female.**

这是一个与我们的小型啮齿动物代谢笼系统一起使用的环境外壳的请求，该系统将允许在代谢笼内进行可变温度控制。这种基础设施是迫切需要的，因为它将使我们能够在一系列温度下测量能量消耗和全身基质氧化，这是我们目前不具备的能力。鉴于我们的NSERC资助的研究计划扩展到寒冷中燃料代谢的研究以及越来越多地认识到在热中性条件下住房啮齿动物的重要性，这种需求变得至关重要;事实上，我们最近的一些论文和资助申请，因未能测量热中性条件下的能量消耗而受到批评。为了使我们保持国际竞争力，并继续为高素质人员（HQP）提供最高标准的培训，我们的研究计划必须能够在一系列环境温度下测量能量消耗和燃料氧化。虽然该地区的其他大学有代谢笼，能够调节温度，但在机构之间转移动物在后勤上具有挑战性，可能会对动物造成压力，这可能会混淆实验结果。此外，实验通常涉及一次监测动物数天，这意味着对系统的要求很高，这进一步限制了在其他机构访问所需基础设施的可行性。 考虑到这些问题，在内部获得所需的基础设施是测量不同温度下能源消耗的唯一可行方法。主要申请人的研究计划是综合性的，鼓励HQP考虑影响生物系统的所有因素。测量能量消耗提供了关于对各种扰动的响应的关键信息。事实上，获得所要求的基础设施将使我们能够考虑在寒冷环境中改变能源消耗和燃料利用。这些相同的终点也将在热中性条件下饲养的实验啮齿动物中进行测量，这种条件越来越被认为上级室温条件下饲养的人体生理学可转换性。来自6个研究实验室的40多名HQP将立即直接受益于所需设备的采购。 该小组包括17名硕士，14名博士，13名本科生和4名博士后研究员，这是一个庞大而多样化的学员群体，来自多个寻求公平的团体。值得注意的是，预期用户实验室中超过50%的HQP是女性。