Regulation of Arabidopsis shikimate pathway

拟南芥莽草酸途径的调控

• 批准号: 250203-2013
• 负责人: Christendat, Dinesh
• 金额: $ 3.19万
• 依托单位: University of Toronto
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=524957
• 关键词: Regulation; Arabidopsis; shikimate; pathway

This application involves the functional annotation of enzymes of the shikimate pathway. The shikimate pathway produces precursors for the synthesis of the aromatic amino acids phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp). They are used by plants to synthesize many aromatic compounds; including phytoalexins, flavonoids, phenylpropanoids, indole hormones and lignin. In plants, these molecules have important biological roles in cellular signaling, pathogen defense, UV protection, and structural support. In addition the shikimate pathway has important therapeutic and biotechnological applications. The pathway is absent in animals, but present in plants and microbes, which makes it an ideal target for herbicides and anti-microbial agents. A variety of shikimate pathway-derived flavonoids and phenylpropanoids have also been shown to have a wide range of health benefits because of their antioxidant properties. Therefore, the overall goal of our research program is to understand how the shikimate pathway is regulated so that we can modulate its properties to achieve desirable end products. We will be focusing on three enzymes, shikimate kinase, shikimate dehydrogenase and DAHP synthase, because they are found to participate in the regulation of the shikimate pathway. There are three enzyme forms of DAHP synthase in Arabidopsis and are found to be differentially regulated by the aromatic amino acids. Four shikimate kinase gene duplicates are found in Arabidopsis and are shown to have evolved distinct biological functions. Similarly, five classes of shikimate dehydrogenases are found in microbes, each of which has a specific biological role.

该应用涉及莽草酸途径酶的功能注释。 莽草酸途径产生合成芳香族氨基酸苯丙氨酸 (Phe)、酪氨酸 (Tyr) 和色氨酸 (Trp) 的前体。它们被植物用来合成许多芳香族化合物；包括植物抗毒素、类黄酮、苯丙素、吲哚激素和木质素。在植物中，这些分子在细胞信号传导、病原体防御、紫外线防护和结构支撑方面具有重要的生物学作用。此外，莽草酸途径具有重要的治疗和生物技术应用。该途径在动物中不存在，但存在于植物和微生物中，这使其成为除草剂和抗微生物剂的理想靶标。多种源自莽草酸途径的类黄酮和苯丙素也因其抗氧化特性而被证明具有广泛的健康益处。 因此，我们研究计划的总体目标是了解如何调节莽草酸途径，以便我们可以调节其特性以获得理想的最终产品。我们将重点关注三种酶：莽草酸激酶、莽草酸脱氢酶和 DAHP 合酶，因为它们被发现参与莽草酸途径的调节。拟南芥中存在三种 DAHP 合酶形式，并且发现它们受到芳香族氨基酸的差异调节。 在拟南芥中发现了四种莽草酸激酶基因重复，并显示出已进化出不同的生物学功能。 同样，在微生物中发现了五类莽草酸脱氢酶，每种都具有特定的生物学作用。