Transcriptional regulation of zebrafsh forebrain patterning

斑马鱼前脑模式的转录调控

• 批准号: 298371-2010
• 负责人: Waskiewicz, Andrew
• 金额: $ 3.35万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526002
• 关键词: Transcriptional; regulation; zebrafsh; forebrain; patterning

Regional cell identity in the early vertebrate embryo is created, in part, by a series of organizers, which secrete signaling molecules and specify the identity of surrounding cells. The prechordal plate is the anterior-most axial mesoderm and is a critical organizer that underlies the forebrain and specifies ventral midline identity. Embryos that lack a prechordal plate do not express markers of ventral forebrain identity or separate left and right cerebral hemispheres. The effects of this are profound, including failure of retinal cell migration, leading to the formation of a single cyclopic eye. Animal model studies have identified two signaling pathways - Sonic Hedgehog (Shh) and Retinoic Acid (RA) - that are critically important for specification and function of the prechordal plate.

早期脊椎动物胚胎中的区域细胞身份部分是由一系列组织者创造的，这些组织者分泌信号分子并指定周围细胞的身份。前索板是最前面的轴向中胚层，是一个重要的组织者，位于前脑的下方，并指定腹侧中线的身份。缺乏前索板的胚胎不表达腹侧前脑身份或分离的左，右大脑半球的标记。这种影响是深远的，包括视网膜细胞迁移的失败，导致形成一个单一的独眼。动物模型研究已经确定了两个信号通路-- Sonic Hedgehog（Shh）和视黄酸（RA）--它们对腱前板的规格和功能至关重要。