Regulation of solute and water balance in primitive fishes

原始鱼类溶质和水平衡的调节

• 批准号: 311909-2010
• 负责人: Anderson, WGary
• 金额: $ 1.97万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=526304
• 关键词: Regulation; solute; water; balance; primitive

Endocrine control of solute and water balance is a life sustaining process in all organisms. It requires the coordinated action of a number of different physiological systems through the interaction of a multitude of hormones. Hormones such as angiotensin II, C-type natriuretic peptide, bradykinin and arginine vasotocin are all involved in solute and water regulation in mammals. Research has shown that these hormones also exist, and have physiological function, in the phylogenetically ancient elasmobranch fish. The proposed research programme will examine the role these hormones play in the regulation of renal, cardiovascular and gastro-intestinal physiology of elasmobranch fish. In addition, molecular characterisation of key transport proteins for urea and ammonia will provide new information on nitrogen balance in elasmobranchs. Furthermore, the role ureolytic bacteria may play in nitrogen metabolism in these primitive fish will be examined. The proposed research is significant as it aims to provide an evolutionary framework for solute transport mechanisms in vertebrates and a physiological understanding of how hormone systems regulate similar physiological functions in elasmobranch fish. Additional research is proposed to examine calcium transport and the endocrine regulation of this key ion in a second primitive fish, the lake sturgeon. These freshwater cartilaginous fish present a unique model in terms of calcium regulation. Molecular characterisation of transport mechanisms in addition to hormonal regulation of this ion will aid in our understanding of calcium metabolism in these fish and therefore the proposed research will aid in the mitigation of potential impacts in the documented declines of environmental calcium may have on this threatened species. Therefore, information gained from this proposed research programme can be used not only in the context of industry as this fundamental research underpins more advanced and innovative research in the development of therapeutics in the pharmaceutical industry but also from the context of conservation physiology for the protection of threatened species such as the lake sturgeon.

溶质和水平衡的内分泌控制是所有生物体的生命维持过程。它需要通过多种激素的相互作用来协调许多不同的生理系统的作用。血管紧张素II、C型利尿钠肽、缓激肽和精氨酸加压素等激素都参与哺乳动物的溶质和水调节。研究表明，这些激素在系统发育古老的软骨鱼类中也存在，并具有生理功能。拟议的研究计划将研究这些激素在软骨鱼肾脏、心血管和胃肠道生理调节中的作用。此外，尿素和氨关键转运蛋白的分子表征将为软骨鱼氮平衡提供新信息。此外，还将研究尿素分解细菌在这些原始鱼类的氮代谢中可能发挥的作用。这项研究意义重大，因为它旨在为脊椎动物溶质运输机制提供进化框架，并从生理学角度理解激素系统如何调节软骨鱼类的类似生理功能。还提出了进一步的研究来检查第二种原始鱼类湖鲟的钙运输和这种关键离子的内分泌调节。这些淡水软骨鱼在钙调节方面呈现出独特的模式。除了该离子的激素调节之外，运输机制的分子表征将有助于我们了解这些鱼类的钙代谢，因此拟议的研究将有助于减轻已记录的环境钙下降可能对该受威胁物种产生的潜在影响。因此，从这项拟议的研究计划中获得的信息不仅可以在工业背景下使用，因为这项基础研究支持制药行业治疗方法开发中更先进和创新的研究，而且还可以在保护生理学背景下使用，以保护湖鲟等受威胁物种。