Roles and molecular mechanisms of SUMO-interaction motifs and SUMOylation of retroviral integrase in mediating its cofactor binding and beyond

SUMO相互作用基序的作用和分子机制以及逆转录病毒整合酶的SUMO化在介导其辅因子结合及其他方面的作用和分子机制

• 批准号: 418543-2013
• 负责人: Yao, Xiaojian
• 金额: $ 2.62万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=531235
• 关键词: Roles; molecular; mechanisms; SUMO; interaction

SUMOylation is an important posttranslational modification of proteins that regulates their biological activities and cross-talking with their cofactors. It is known that SUMOylated proteins are able to bind to a SUMO-interaction motif (SIMs) present in their cofactors and such cross-talking between different proteins links different signal pathways and regulate their biological fucntions. HIV-1 integrase has recently been shown to be a SUMOylated protein. Also, such putative SUMOylation sites were also found in different animal retroviral integrases. However, a complete mapping and understanding of how SUMOylation could regulate their biological actions still remain elusive. Interestingly, our sequence analysis further revealed three SIMs present in this retroviral protein, suggesting that the SUMOylation and SIMs present in the same protein may coordinate together and regulate its communication with other cellular cofactors.

Sumoylation是对蛋白质的重要​​后翻译后修饰，可调节其生物学活性并与辅因子进行串扰。众所周知，sumoylated蛋白能够结合其辅助因子中存在的相互作用基序（SIMS），并且不同蛋白之间的这种交叉言语连接不同的信号途径并调节其生物学渠道。 HIV-1积分酶最近已显示为Sumoylated蛋白。同样，在不同的动物逆转录病毒整合酶中也发现了这种推定的Sumoylation位点。 但是，对Sumoylation如何调节其生物行为的完整映射和理解仍然难以捉摸。有趣的是，我们的序列分析进一步揭示了该逆转录病毒蛋白中存在的三个SIMS，这表明同一蛋白质中存在的sumoylation和SIMS可以将其协调并调节其与其他细胞辅助因子的通信。