A Total Internal Reflection Fluorescence (TIRF) Microscope for the Maritimes

适用于海洋的全内反射荧光 (TIRF) 显微镜

• 批准号: 458602-2014
• 负责人: McCormick, Craig
• 金额: $ 3.9万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=541981
• 关键词: Total; Internal; Reflection; Fluorescence; TIRF

Powerful new techniques now allow researchers to attach fluorescent tags to individual molecules and track their movement and activity inside a living cell. The utility of this technology is only limited by the method of detection; not every microscope is equipped to track individual fluorescent molecules. TIRF microscopy allows for superior visualization of single molecules inside a living cell. We propose to upgrade an existing microscope to enable TIRF microscopy. This cost-effective approach will greatly advance the research productivity of eight researchers and their trainees. As the only TIRF-enabled microscope in the Maritimes, we anticipate that it will become a regional resource for academic research and training. This technology will be applied to understand how proteins interact with each other or respond to different treatments, and how these proteins work alone or together with other proteins to regulate or alter the normal function of cells. Studies performed with this live cell imaging system will provide important insights into normal cellular function and how cells respond to various stresses.

强大的新技术现在允许研究人员将荧光标签附加到单个分子上，并跟踪它们在活细胞内的运动和活动。该技术的实用性仅受检测方法的限制；并不是每台显微镜都配备了追踪单个荧光分子的设备。TIRF显微镜允许在活细胞内的单个分子的优越的可视化。我们建议将现有的显微镜升级为TIRF显微镜。这种具有成本效益的方法将大大提高8名研究人员及其学员的研究生产力。作为海事地区唯一一台启用tirf的显微镜，我们预计它将成为学术研究和培训的区域资源。这项技术将被应用于了解蛋白质如何相互作用或对不同的治疗作出反应，以及这些蛋白质如何单独或与其他蛋白质一起调节或改变细胞的正常功能。使用这种活细胞成像系统进行的研究将为正常细胞功能和细胞如何应对各种压力提供重要的见解。