Advanced Neurostereology System for Integrative Neurobiology Group

这是一项修订后的申请，目的是获得资金，以升级现有的显微镜设备，以适应西安大略大学一组综合神经生物学家的先进神经体视学系统。一组由NSERC资助的实验室试图用MicroBrightfield的新彩色成像相机和Stereo Investigator系统升级共享的尼康Eclipse 90i显微镜。这一最先进的神经体视学系统将提供一种经济高效的解决方案，以获得对脑组织切片中细胞或生物结构的数量、长度、面积和体积的准确、无偏见的估计。MicroBrightfield的立体声调查系统是世界上最先进的神经体视学系统。无论是这个系统，还是任何类似的系统，都不适用于WESTERN或安大略省伦敦市的任何其他机构。这项请求中包括一个扩展模块，以便每个使用通用数据采集系统的研究实验室都可以在显微镜之外进行立体分析。这将大大提高普通显微镜的效率，因为使用显微镜进行数据采集不会阻止其他研究人员进行数据分析。总而言之，这个数据采集和分析系统的完成将是我们联合研究计划的一个重大进步，并将使我们所有由NSERC赞助的项目受益。WESTERN的综合神经生物学小组包括多个系(心理学、生理学和药理学、解剖学和细胞生物学以及听力学)和多个系(社会科学、医学和牙科以及健康科学)的实验室。HQP将在六个不同的实验室各级使用这些设备，以及其他实验室的受训人员，这些受训人员将能够根据需要使用设备。总体而言，购买这种设备将对可以推进的研究和许多研究学科可获得的培训机会的广度产生直接和长期的影响。

This is a revised application to obtain funds to upgrade existing microscopy equipment to accommodate an advanced neurostereology system for a group of integrative neurobiologists at the University of Western Ontario. A group of NSERC-funded labs seeks to upgrade a shared Nikon Eclipse 90i microscope with a new colour imaging camera and a Stereo Investigator system from MicroBrightField. This state-of-the-art advanced neurostereology system will provide a cost-effective solution to acquire accurate, unbiased estimates of the number, length, area, and volume of cells or biological structures in a brain tissue section. The Stereo Investigator system from MicroBrightField is the world's foremost system for advanced neurostereology. Neither this system, nor any similar system, is available at Western or from any other institution in the London, Ontario area. Included in this request is an extension module so that each research lab using the common data acquisition system can perform their stereological analysis away from the microscope. This will greatly improve the efficiency of the common microscope, as microscope use for data acquisition will not prevent other researchers from performing data analysis. In total, completion of this data acquisition and analysis system will be a significant advance for our combined research programs and will benefit all of our NSERC-sponsored projects. The Integrative Neurobiology Group at Western includes laboratories across multiple departments (Psychology, Physiology and Pharmacology, Anatomy & Cell Biology, and Audiology) and multiple faculties (Social Science, Medicine & Dentistry, and Health Science). This equipment will be utilized by HQP at all levels, across six different laboratories, as well as trainees from other laboratories that will be able to access the equipment as needed. Overall, the acquisition of this equipment will have both an immediate and long-lasting effect on the research that can be advanced and on the breadth of the training opportunities available in many research disciplines.