Electrospray-Differential Mobility Analysis System for the Analysis of Biomacromolecules, Polymeric (Bio)Materials and Nanoparticles

为了有效地开展他们在胶体和聚合物科学、软物质、微流体和再生医学方面的研究项目，蒙特利尔理工学院的一组研究人员迫切需要一种分析工具，能够快速表征不同种类的复杂纳米体系(生物分子、聚合物和纳米颗粒)的大小和浓度。这项提案的申请人已经确定了一种分析方法，用于快速和准确地表征这类系统，即电喷雾-差示迁移率分析(ES-DMA)。本方案中要求的ES-DMA仪器能够在几分钟内测定分子质量和尺寸分别从~8 kDa到~gt；100 mda和从2.5到1000 nm的大分子和纳米颗粒的数量、浓度和尺寸分布。ES-DMA方法是最近开发的，目前魁北克的学术界和工业界还不能使用。申请者的小组成员和当地科学界完全和不受阻碍地访问ES-DMA将通过克服不同种类纳米系统带来的大小表征挑战，极大地加快其研究计划的进展。

To effectively carry out their research projects in colloidal and polymer science, soft matter, microfluidics, and regenerative medicine, a group of researchers from Ecole Polytechnique de Montreal urgently require an analytical tool that can rapidly characterize heterogeneous complex nanosized systems (biomolecules, polymers and nanoparticles) for their the size and concentration. The applicants of this proposal have identified an analytical method for rapid and precise characterization of such systems, namely Electrospray-Differential Mobility Analysis (ES-DMA). The ES-DMA instrument requested in this proposal is able to determine number concentration and size distribution of macromolecules and nanoparticles with molecular weight and size ranging from ~8 kDa to > 100 MDa and from 2.5 to 1000 nm, respectively, within a few minutes. The ES-DMA method has been recently developed and is not currently available to the academic and industrial community in Quebec. A complete and unhindered access of the applicants' group members and the local scientific community at large to ES-DMA will greatly accelerate progress in their research programs by overcoming the size characterization challenge posed by heterogeneous nanosized systems.