Electrospray-Differential Mobility Analysis System for the Analysis of Biomacromolecules, Polymeric (Bio)Materials and Nanoparticles

用于分析生物大分子、聚合（生物）材料和纳米粒子的电喷雾差动迁移率分析系统

• 批准号: 458788-2014
• 负责人: Buschmann, Michael
• 金额: $ 10.9万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2013
• 资助国家: 加拿大
• 起止时间: 2013-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=542037
• 关键词: Electrospray; Differential; Mobility; Analysis; System

To effectively carry out their research projects in colloidal and polymer science, soft matter, microfluidics, and regenerative medicine, a group of researchers from Ecole Polytechnique de Montreal urgently require an analytical tool that can rapidly characterize heterogeneous complex nanosized systems (biomolecules, polymers and nanoparticles) for their the size and concentration. The applicants of this proposal have identified an analytical method for rapid and precise characterization of such systems, namely Electrospray-Differential Mobility Analysis (ES-DMA). The ES-DMA instrument requested in this proposal is able to determine number concentration and size distribution of macromolecules and nanoparticles with molecular weight and size ranging from ~8 kDa to > 100 MDa and from 2.5 to 1000 nm, respectively, within a few minutes. The ES-DMA method has been recently developed and is not currently available to the academic and industrial community in Quebec. A complete and unhindered access of the applicants' group members and the local scientific community at large to ES-DMA will greatly accelerate progress in their research programs by overcoming the size characterization challenge posed by heterogeneous nanosized systems.

为了有效地开展他们在胶体和聚合物科学，软物质，微流体和再生医学方面的研究项目，蒙特利尔理工学院的一组研究人员迫切需要一种分析工具，可以快速表征异质复杂纳米系统（生物分子，聚合物和纳米颗粒）的大小和浓度。该提案的申请人已经确定了一种用于快速和精确表征此类系统的分析方法，即电喷雾-微分迁移率分析（ES-DMA）。本提案中要求的ES-DMA仪器能够在几分钟内分别测定分子量和尺寸范围为约8 kDa至> 100 MDa和2.5至1000 nm的大分子和纳米颗粒的数量浓度和尺寸分布。ES-DMA方法是最近开发的，目前还没有提供给魁北克的学术界和工业界。申请人的小组成员和当地科学界对ES-DMA的完全和不受阻碍的访问将通过克服异质纳米系统所带来的尺寸表征挑战来大大加速其研究计划的进展。