Production of chitosan/nucleic acid nanoparticles

壳聚糖/核酸纳米颗粒的生产

• 批准号: 445241-2012
• 负责人: Buschmann, Michael
• 金额: $ 13.1万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=554151
• 关键词: Production; chitosan; nucleic; acid; nanoparticles

Polyelectrolyte complexes are nanoparticles that form via electrostatic attraction between a polycation and polyanion. A particular class of these systems is used to introduce genetic material into cells when the polyanion is a nucleic acid sequence like DNA or RNA. We have studied these delivery systems using the polycation chitosan, a natural polymer of glucosamine that is partly acetylated, and plasmid DNA to express genes when delivered to the nucleus. We have also developed nanoparticles containing siRNA which stop expression of the target gene. Patent-pending formulations of these nucleic acid delivery systems have commercial potential as transfection reagents to express proteins in cells or to silence genes through RNA interference and also as therapeutic agents that can be applied to animals and humans for different diseases.

聚电解质复合物是通过聚阳离子和聚苯翁之间的静电吸引力形成的纳米颗粒。当聚会是DNA或RNA等核酸序列时，这些系统的特定类别用于将遗传物质引入细胞中。我们已经使用多阳离子壳聚糖（一种自然的葡萄糖聚合物聚合物）研究了这些递送系统，该聚合物是部分乙酰化的，而质粒DNA则在传递到核时表达基因。我们还开发了含有siRNA的纳米颗粒，这些siRNA停止了靶基因的表达。这些核酸输送系统的专利制剂具有转染试剂的商业潜力，以表达细胞中的蛋白质或通过RNA干扰沉默基因，也可以作为可以应用于动物和人类的不同疾病的治疗剂。