Production of chitosan/nucleic acid nanoparticles

壳聚糖/核酸纳米颗粒的生产

• 批准号: 445241-2012
• 负责人: Buschmann, Michael
• 金额: $ 13.1万
• 依托单位: École Polytechnique de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=554151
• 关键词: Production; chitosan; nucleic; acid; nanoparticles

Polyelectrolyte complexes are nanoparticles that form via electrostatic attraction between a polycation and polyanion. A particular class of these systems is used to introduce genetic material into cells when the polyanion is a nucleic acid sequence like DNA or RNA. We have studied these delivery systems using the polycation chitosan, a natural polymer of glucosamine that is partly acetylated, and plasmid DNA to express genes when delivered to the nucleus. We have also developed nanoparticles containing siRNA which stop expression of the target gene. Patent-pending formulations of these nucleic acid delivery systems have commercial potential as transfection reagents to express proteins in cells or to silence genes through RNA interference and also as therapeutic agents that can be applied to animals and humans for different diseases.

聚电解质复合物是通过聚阳离子和聚阴离子之间的静电吸引形成的纳米颗粒。当聚阴离子是核酸序列如DNA或RNA时，这些系统中的特定类别用于将遗传物质引入细胞中。我们研究了这些传递系统使用聚阳离子壳聚糖，一种天然聚合物的葡萄糖胺，部分乙酰化，和质粒DNA表达基因时，交付到细胞核。我们还开发了含有siRNA的纳米颗粒，可以阻止靶基因的表达。这些核酸递送系统的正在申请专利的制剂具有作为转染试剂的商业潜力，以在细胞中表达蛋白质或通过RNA干扰使基因沉默，并且还可以作为治疗剂应用于动物和人类的不同疾病。