Tetrad Dissection Microscope for Yeast Genetics Analyses

用于酵母遗传学分析的四分体解剖显微镜

• 批准号: 472402-2015
• 负责人: Rosonina, Emanuel
• 金额: $ 1.35万
• 依托单位: York University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564743
• 关键词: Tetrad; Dissection; Microscope; Yeast; Genetics

Because it is easy to grow in the lab and manipulate genetically, biologists have used yeast extensively in the past several decades to make important discoveries about cellular processes, many of which are common to humans and other eukaryotic organisms. In the post-genomic era, through vast and comprehensive genome-wide genetic analyses, yeast has played a central role in rapidly advancing our knowledge of how organisms coordinate the expression of their genes and respond to stress. The Department of Biology at York University now has two labs that use yeast as their primary model organism. To fully benefit from the potential that working with this organism provides, and towards developing a fully-equipped yeast genetics facility at the university, we are requesting funds to acquire a tetrad dissection microscope. This instrument, which is an indispensable tool for labs working with yeast, is used for generating specific mutant strains, for examining the growth phenotypes of these strains, and for uncovering novel genetic interactions that reveal relationships between biological pathways.

因为它很容易在实验室中生长并进行遗传操作，生物学家在过去几十年中广泛使用酵母来发现细胞过程的重要发现，其中许多是人类和其他真核生物所共有的。在后基因组时代，通过广泛而全面的全基因组遗传分析，酵母在快速推进我们对生物体如何协调其基因表达和应对压力的认识方面发挥了核心作用。约克大学生物系现在有两个实验室使用酵母作为他们的主要模式生物。为了充分利用与这种生物体合作所提供的潜力，并在大学建立一个设备齐全的酵母遗传学设施，我们正在申请资金以获得四分体解剖显微镜。该仪器是酵母实验室不可或缺的工具，用于产生特定的突变菌株，检查这些菌株的生长表型，并揭示揭示生物途径之间的关系的新的遗传相互作用。