Shaker incubators for cultivation of bacteria

用于细菌培养的摇床培养箱

• 批准号: 472653-2015
• 负责人: Zechel, David
• 金额: $ 7.23万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564804
• 关键词: Shaker; incubators; cultivation; bacteria

Bacteria, yeasts, and other microbes have a long history of providing beneficial services and products to mankind. This includes fermentation (beer, wine), food production (bread, cheese), and production of lifesaving drugs (penicillin). This process of domesticating microbes for specific applications continues to this day, but now occurs at a molecular level. Today, the genes within the microbe are specifically altered to program the organism to perform a specific task. This may be done to learn more about the functions of genes in the organism, or to program the microorganism to produce useful molecules for study, such as proteins or drugs. This equipment proposal centres around six shaker-incubators, which will be used by graduate students from four different labs at Queen's University to cultivate microbes under controlled conditions. This will allow the graduate students to study bacteria that produce drug-like molecules as well as to produce proteins in bacteria in order to understand their function in disease and cell processes. For example, graduate students will use the shakers to access new antibiotics from soil and marine bacteria in order to address the rise of antibiotic resistance to our current drugs. Students will also produce proteins from pathogenic bacteria in order to understand how these proteins contribute to diseases caused by these bacteria. Finally, the shakers will be used to produce unique enzymes discovered through genome sequencing in order to understand their mechanisms. This will enable the development of these enzymes as environmentally friendly industrial catalysts and as agents for degrading pollutants found in soil and water bodies.

细菌、酵母和其他微生物在为人类提供有益的服务和产品方面有着悠久的历史。这包括发酵（啤酒，葡萄酒），食品生产（面包，奶酪）和救生药物（青霉素）的生产。这种为特定应用而驯化微生物的过程一直持续到今天，但现在发生在分子水平上。今天，微生物内的基因被专门改变，以编程生物体执行特定任务。这样做可以更多地了解生物体中基因的功能，或者对微生物进行编程以产生有用的分子用于研究，例如蛋白质或药物。该设备提案围绕六个振荡培养箱展开，将由皇后大学四个不同实验室的研究生在受控条件下培养微生物。这将使研究生能够研究产生药物样分子的细菌，以及在细菌中产生蛋白质，以了解它们在疾病和细胞过程中的功能。例如，研究生将使用振动筛从土壤和海洋细菌中获得新的抗生素，以解决抗生素对我们现有药物的耐药性上升问题。学生还将从病原菌中产生蛋白质，以了解这些蛋白质如何导致这些细菌引起的疾病。最后，振动器将用于生产通过基因组测序发现的独特酶，以了解它们的机制。这将使这些酶能够发展成为环境友好的工业催化剂和降解土壤和水体中污染物的试剂。