HPLC-DAD-MS system

HPLC-DAD-MS系统

• 批准号: 422374-2012
• 负责人: Zechel, David
• 金额: $ 10.93万
• 依托单位: Queen's University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2011
• 资助国家: 加拿大
• 起止时间: 2011-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=495778
• 关键词: HPLC; DAD; MS; system

The HPLC-DAD-MS system is an analytical instrument that can isolate molecules from a complex mixture and subsequently identify unique spectroscopic features for each molecule. Such data is tremendously useful for the analysis of the products of chemical reactions and the contents of bioactive extracts derived from natural sources. This fully automated instrument can also process dozens of samples each day. For these reasons HPLC-DAD-MS systems are widely used in pharmaceutical and biotechnology companies. To better prepare our graduate students for the workplace, the HPLC-DAD-MS system described in this proposal will be used by 56 students from five research groups to obtain extensive hands-on training and experience, as well as accelerate their research in a number of programs. This includes the biosynthesis of drug-like molecules by soil bacteria, enzyme catalysis, repair of damaged DNA with artificial receptors, extraction of oils using green solvent systems, the development of miniature analytical devices, and the development of a novel reaction for the synthesis of complex molecules.

HPLC-DAD-MS系统是一种分析仪器，可以从复杂的混合物中分离分子，然后识别每个分子的独特光谱特征。这些数据对于分析化学反应产物和来自天然来源的生物活性提取物的含量非常有用。这台全自动仪器每天还可以处理数十个样品。由于这些原因，高效液相色谱-DAD-MS系统被广泛应用于制药和生物技术公司。为了让我们的研究生更好地为工作做好准备，来自五个研究小组的56名学生将使用本提案中描述的高效液相-DAD-MS系统，以获得广泛的实践培训和经验，并加快他们在多个项目中的研究。这包括土壤细菌生物合成类药物分子，酶催化，用人工受体修复受损的DNA，使用绿色溶剂系统提取油，开发微型分析设备，以及开发合成复杂分子的新反应。