Fast Time-Resolved Fluorometer

快速时间分辨荧光计

• 批准号: 472643-2015
• 负责人: Duhamel, Jean
• 金额: $ 10.93万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=564802
• 关键词: Fast; Time; Resolved; Fluorometer

This proposal is a request to purchase a fast time-resolved fluorometer. Fluorescence describes the process whereby a dye releases its excess energy by emitting a photon. This process occurs on a time scale of a few tens of nanoseconds and a time-resolved fluorometer (TRF) measures how quickly the fluorescence emitted by a dye dissipates over time. TRFs are used to study a variety of molecules which are used in plastics, detergents, proteins, DNA, and many other synthetic and natural products. Many fluorescence studies probe the interactions between a dye and another molecule that is introduced in the system to quench the fluorescence of the dye. The time scale over which quenching occurs provides critical information about the system under study. For instance, the low quenching rate of a dye located in the hydrophobic pocket of a macromolecule by a water-soluble quencher demonstrates that the interior of the hydrophobic pocket is highly viscous locally and that the macromolecule could be used as a delivery vehicle for the slow release of hydrophobic drugs.

此建议是一个请求购买快速时间分辨荧光计。荧光描述了染料通过发射光子释放其多余能量的过程。这个过程发生在几十纳秒的时间尺度上，时间分辨荧光计（TRF）测量染料发出的荧光随时间消散的速度。TRFs用于研究塑料，洗涤剂，蛋白质，DNA和许多其他合成和天然产品中使用的各种分子。许多荧光研究探测染料与引入系统中以淬灭染料荧光的另一分子之间的相互作用。猝灭发生的时间尺度提供了关于所研究系统的关键信息。 例如，位于大分子疏水口袋中的染料被水溶性猝灭剂的低猝灭速率表明疏水口袋的内部局部是高度粘性的，并且大分子可以用作疏水性药物缓慢释放的递送载体。