Simultaneous imaging of protein-protein and protein-membrane interactions using molecular rotors

使用分子转子同时成像蛋白质-蛋白质和蛋白质-膜相互作用

• 批准号: 2741582
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2741582
• 关键词: Simultaneous; imaging; protein; membrane; interactions

Protein-protein interactions (PPIs) are integral to all biological processes, including those involved in disease pathways. Methods to study and quantify the effect of targeting these interactions in real time and at a single cell level are currently lacking. Molecular rotors, environmentally sensitive fluorophores, offer an exciting opportunity to quantitatively detect the function of a PPI inhibitor as a large change in fluorescence lifetime. We will develop novel peptide-rotor conjugates as tools to study the Bcl-2 family of PPIs, key oncology targets. Inhibition of these PPIs is inexorably linked to permeabilisation of the mitochondrial outer membrane. By employing rotors with complementary emission properties, we will study both PPI inhibition and membrane integrity simultaneously, offering unprecedented insight into these interlinked biological events.

蛋白质-蛋白质相互作用（PPI）是所有生物过程的组成部分，包括参与疾病途径的生物过程。目前缺乏在真实的时间和单细胞水平上研究和量化靶向这些相互作用的效果的方法。分子转子，环境敏感的荧光团，提供了一个令人兴奋的机会，定量检测PPI抑制剂的功能作为荧光寿命的大变化。我们将开发新的肽转子共轭物作为工具，研究Bcl-2家族的PPI，关键的肿瘤学目标。这些PPI的抑制与线粒体外膜的透化密切相关。通过采用具有互补发射特性的转子，我们将同时研究PPI抑制和膜完整性，为这些相互关联的生物事件提供前所未有的见解。