Regulatory control of genes associated with cyst biogenesis in Legionella pneumophila

嗜肺军团菌包囊生物发生相关基因的调控

• 批准号: RGPIN-2014-03941
• 负责人: Brassinga, AnnKaren
• 金额: $ 2.55万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2014
• 资助国家: 加拿大
• 起止时间: 2014-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=566189
• 关键词: Regulatory; control; genes; associated; cyst

In water and moist soil environments, unicellular organisms (termed protozoa) eat bacteria for food. One type of bacterium, Legionella, is a parasite of protozoa and when ingested, uses the protozoan host cell as a food source for its lifecycle. The Legionella lifecycle is unique because it alternates between two bacterial forms: the replicative form (RF) and the cyst-like form (CLF). When food is abundant, RFs actively replicate themselves and when the bacteria sense that food is becoming scarce, the RFs differentiate into CLFs, a dormant form that has fortified cell walls to ensure survival in the environment. The spent protozoan host cell bursts open releasing the CLFs into the environment where they will be ingested by foraging protozoa repeating the life-cycle. The CLFs are much more infectious than the RFs, and it is currently believed that when CLFs are aerosolized in water droplets and inadvertently inhaled by humans, they cause a respiratory infection called Legionnaires' disease (LD). This research is intended to investigate the molecular regulatory mechanisms controlling CLF formation of which the results will lead to improved control measures and treatment strategies. Reservoirs of Legionella bacteria include natural and man-made freshwater systems, and recently it was discovered that Legionella bacteria can undergo their lifecycle in soil nematodes normally present in garden potting soil. This research will further examine the Legionella life-cycle in nematodes to investigate whether nematodes can retain and disperse Legionella bacteria within the soil. If this is the case, then free-living nematodes may serve both as reservoir and an amplifier of Legionella in the natural environment. This ecological factor was not previously considered in the etiology of Legionnaire's disease, and thereby investigating the role of the nematode host will have a dramatic impact on the current understanding of the Legionella bacteria life-style.

在水和潮湿的土壤环境中，单细胞生物（称为原生动物）以细菌为食物。一种细菌，军团菌，是原生动物的寄生虫，当摄入时，使用原生动物宿主细胞作为其生命周期的食物来源。军团菌的生命周期是独特的，因为它在两种细菌形式之间交替：复制形式（RF）和囊肿样形式（CLF）。当食物丰富时，RF会主动复制自己，当细菌感觉到食物变得稀缺时，RF会分化为CLF，这是一种休眠形式，具有强化的细胞壁以确保在环境中生存。消耗的原生动物宿主细胞爆裂，将CLF释放到环境中，在那里它们将被觅食的原生动物摄取，重复生命周期。CLF比RF更具传染性，目前认为，当CLF在水滴中雾化并被人类无意中吸入时，它们会引起称为军团病（LD）的呼吸道感染。本研究旨在探讨控制CLF形成的分子调控机制，其结果将导致改进的控制措施和治疗策略。军团菌的宿主包括天然和人造淡水系统，最近发现军团菌可以在通常存在于花园盆栽土壤中的土壤线虫中经历其生命周期。本研究将进一步研究军团菌在线虫体内的生命周期，以研究线虫是否能在土壤中保留和分散军团菌。如果是这种情况，那么自由生活的线虫可能既是自然环境中军团菌的储存者，也是军团菌的放大者。以前在军团病的病因学中没有考虑到这种生态因素，因此研究线虫宿主的作用将对目前对军团菌生活方式的了解产生巨大影响。