Regulation Of Protein Metabolism By Cellular Signaling Pathways In Stressed Fish

应激鱼中细胞信号通路对蛋白质代谢的调节

• 批准号: 435638-2013
• 负责人: Lamarre, Simon
• 金额: $ 2.19万
• 依托单位: Université de Moncton
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=574133
• 关键词: Regulation; Protein; Metabolism; Cellular; Signaling

Animals produce soft tissues by synthesizing proteins and retaining a proportion as protein growth; the remainder being degraded and the amino acids recycled. These interrelated processes of protein synthesis, protein growth and protein degradation are collectively called protein metabolism. In fish, protein metabolism is highly sensitive to a wide variety of biotic and abiotic factors including temperature, pollution, food availability and probably to the individual's social status. Protein metabolism is highly regulated and, at the cellular level, this regulation involves the signal transduction pathways. In the last decade, our understanding of the roles of the signal transduction pathways in the control of protein metabolism has greatly progressed however much remains to be understood, especially in non-mammalian animals. The long-term goals of my research program are; 1) to characterize the cellular mechanisms involved in the control of protein metabolism and 2) to use these cellular mechanisms to extract information on the physiological status of animals that are either farmed or collected from the wild. In the next five years, I will apply a multidisciplinary approach to study the control of protein metabolism using the growth depression observed in subordinate fish following the establishment of social hierarchy as a model. This growth depression is primarily caused by a dramatic reduction of food consumption however the stress hormone cortisol also plays a role in it. The short term objectives of the proposed research activities are 1) To characterize the cellular mechanisms involved in the adjustments of protein metabolism during starvation, 2) To characterize the effects of acutely and chronically elevated cortisol on protein metabolism, 3) To evaluate the respective contribution of starvation and chronically elevated cortisol on growth depression in subordinate fish. This research program addresses an important aspect of fish physiology that is highly relevant to current industrial (aquaculture) and environmental issues.

动物通过合成蛋白质并保留一定比例的蛋白质生长来产生软组织;其余的被降解，氨基酸被回收。这些相互关联的蛋白质合成、蛋白质生长和蛋白质降解过程统称为蛋白质代谢。在鱼类中，蛋白质代谢对各种生物和非生物因素高度敏感，包括温度、污染、食物供应，可能还对个体的社会地位高度敏感。 蛋白质代谢受到高度调节，在细胞水平上，这种调节涉及信号转导途径。 在过去的十年中，我们对信号转导通路在蛋白质代谢控制中的作用的理解有了很大的进展，但仍有许多有待理解，特别是在非哺乳动物中。 我的研究计划的长期目标是：1）表征参与蛋白质代谢控制的细胞机制，2）使用这些细胞机制来提取有关养殖或从野外收集的动物的生理状态的信息。 在未来的五年里，我将应用多学科的方法来研究蛋白质代谢的控制，使用在从属鱼类中观察到的生长抑制作为模型，随着社会等级的建立。这种生长抑制主要是由食物消耗的急剧减少引起的，然而应激激素皮质醇也在其中起作用。拟议研究活动的短期目标是：1）表征饥饿期间蛋白质代谢调节所涉及的细胞机制，2） 研究急性和慢性皮质醇升高对蛋白质代谢的影响。3）研究饥饿和慢性皮质醇升高对次级鱼类生长抑制的影响。 该研究计划涉及鱼类生理学的一个重要方面，与当前的工业（水产养殖）和环境问题高度相关。