Protein and amino acid metabolism in fish

拟议研究计划的长期目标是在生物化学和分子水平上更好地描述和理解水生生物如何应对环境挑战。动物能量学经过精心调整，以确保在大多数情况下能源供应与能源需求相匹配。这种微妙的平衡可能会受到几个生物和非生物参数的影响，包括温度、污染、营养等。在所有情况下，以ATP形式存在的能量被重新引导到即时的救生功能上，而非必要的功能则暂停，直到有利条件恢复。现在已经很清楚，蛋白质新陈代谢是这样一个过程，它对细胞能量平衡非常敏感。短期目标是：i)表征低氧循环后的表型调整；ii)表征能量需求增加情况下蛋白质代谢的动态；iii)评估S-腺苷蛋氨酸通过一种新发现的连接一碳代谢和蛋白质代谢的信号通路在蛋白质代谢调控中的意义。拟议的工作很重要，因为它侧重于进化的两个重要驱动因素，即能源供需之间的匹配和甲基的提供。这一计划的优势在于它的独创性和在各种代谢挑战中产生关于控制蛋白质代谢的机制的新的重要知识的潜力。这些知识将直接应用于各个领域，特别是在水产养殖、营养和养护生理学方面。

The long-term goal of the proposed research program is to better characterize and understand how aquatic organisms deal with environmental challenges at the biochemical and molecular level. Animal energetics is exquisitely tuned to ensure that energy supply matches energy demand in most situations. This fine balance may be tipped by several biotic and abiotic parameters including temperature, pollution, nutrition, etc. In all cases, energy in the form of ATP is redirected to immediate life-saving functions while non-essential functions are paused until favorable conditions return. It has now become clear that protein metabolism is one such process that is extremely responsive to cellular energy balance. The short-term objectives are to i) characterize the phenotypic adjustments following diel cycling hypoxia, ii) characterize the dynamics of protein metabolism in situations where energy demand is elevated and iii) Evaluate the implication of s-adenosylmethionine in the control of protein metabolism through a newly discovered signalling pathway connecting one-carbon metabolism and protein metabolism. The proposed work is important because it focusses on the two important drivers of evolution that are the match between energy supply and demand and the provision of methyl groups. The strength of this program resides in its originality and potential to generate new important knowledge on the mechanisms controlling protein metabolism during various metabolic challenges. This knowledge will find direct applications in various fields, notably in aquaculture, nutrition and conservation physiology.