Protein and amino acid metabolism in fish

鱼类的蛋白质和氨基酸代谢

• 批准号: RGPIN-2019-05751
• 负责人: Lamarre, Simon
• 金额: $ 2.04万
• 依托单位: Université de Moncton
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=715781
• 关键词: Protein; amino; acid; metabolism; fish

The long-term goal of the proposed research program is to better characterize and understand how aquatic organisms deal with environmental challenges at the biochemical and molecular level. Animal energetics is exquisitely tuned to ensure that energy supply matches energy demand in most situations. This fine balance may be tipped by several biotic and abiotic parameters including temperature, pollution, nutrition, etc. In all cases, energy in the form of ATP is redirected to immediate life-saving functions while non-essential functions are paused until favorable conditions return. It has now become clear that protein metabolism is one such process that is extremely responsive to cellular energy balance. The short-term objectives are to i) characterize the phenotypic adjustments following diel cycling hypoxia, ii) characterize the dynamics of protein metabolism in situations where energy demand is elevated and iii) Evaluate the implication of s-adenosylmethionine in the control of protein metabolism through a newly discovered signalling pathway connecting one-carbon metabolism and protein metabolism. The proposed work is important because it focusses on the two important drivers of evolution that are the match between energy supply and demand and the provision of methyl groups. The strength of this program resides in its originality and potential to generate new important knowledge on the mechanisms controlling protein metabolism during various metabolic challenges. This knowledge will find direct applications in various fields, notably in aquaculture, nutrition and conservation physiology.

拟议研究计划的长期目标是更好地表征和了解水生生物如何在生物化学和分子水平上应对环境挑战。动物能量学被精心调整，以确保在大多数情况下能量供应与能量需求相匹配。这种微妙的平衡可能会被几个生物和非生物参数，包括温度，污染，营养等，在所有情况下，ATP形式的能量被重新定向到立即挽救生命的功能，而非必要的功能被暂停，直到有利的条件返回。现在已经清楚的是，蛋白质代谢是一个对细胞能量平衡非常敏感的过程。短期目标是i）表征昼夜循环缺氧后的表型调整，ii）表征能量需求升高的情况下蛋白质代谢的动力学，和iii）评价s-腺苷甲硫氨酸通过新发现的连接一碳代谢和蛋白质代谢的信号传导途径在控制蛋白质代谢中的意义。拟议的工作很重要，因为它侧重于进化的两个重要驱动因素，即能源供需之间的匹配和甲基的提供。该计划的优势在于其独创性和潜力，以产生在各种代谢挑战中控制蛋白质代谢的机制的新的重要知识。这些知识将直接应用于各个领域，特别是水产养殖，营养和保护生理学。