Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
基本信息
- 批准号:436194-2013
- 负责人:
- 金额:$ 2.55万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Discovery Grants Program - Individual
- 财政年份:2015
- 资助国家:加拿大
- 起止时间:2015-01-01 至 2016-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Transcription factors (TF) are proteins that bind to DNA and regulate the expression of genes. Compared to protein-coding gene mutations, relatively few functional regulatory mutations have been characterized. The overarching goal of this proposal is to identify and determine the function of evolutionarily conserved and species-specific DNA regions that interact with TFs and regulate genes in specific cell types.
Recent technological advances allowing genome-wide experimental isolation of TF-bound DNA sequences have revealed that in any given cell type, there are more TF bound DNA segments than there are genes. Consequentially, this has created a great need for new ways to filter hundreds of thousands of TF-DNA interactions down to the most essential ones that impact gene function.
Comparing DNA between species is a long-established way to identify gene function. Our recent discoveries, comparing TF-DNA interactions in liver tissue from 5 vertebrate species, revealed that conserved TF-DNA interactions significantly enriched for liver TF networks and important conserved liver pathways such as blood clotting factor production and lipid metabolism. There remains, however, a great need to develop a truly multi-species platform that captures normal biological processes, yet is still amenable to experimental manipulation. In this proposal, we will first create a new multi-species TF-DNA interaction atlas in endothelial cells isolated from several mammalian species, including human, dog, mouse, rat and cow. We will then test our prediction that conserved regulatory regions are the important functional ones by performing targeted regulatory DNA deletions. Overall, this work will allow us to make new discoveries regarding the evolution of mammalian gene control.
转录因子(TF)是一种结合DNA并调节基因表达的蛋白质。与蛋白质编码基因突变相比,功能调控突变相对较少。本提案的总体目标是鉴定和确定进化上保守的和物种特异性的DNA区域的功能,这些区域与tf相互作用并调节特定细胞类型中的基因。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Wilson, Michael其他文献
Novel light-activated antimicrobial coatings are effective against surface-deposited Staphylococcus aureus
- DOI:
10.1007/s00284-008-9188-7 - 发表时间:
2008-10-01 - 期刊:
- 影响因子:2.6
- 作者:
Decraene, Valerie;Pratten, Jonathan;Wilson, Michael - 通讯作者:
Wilson, Michael
Plasmodium infection is associated with cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
- DOI:
10.1038/s41598-022-26709-7 - 发表时间:
2022-12-22 - 期刊:
- 影响因子:4.6
- 作者:
Lapidus, Sarah;Liu, Feimei;Casanovas-Massana, Arnau;Dai, Yile;Huck, John D.;Lucas, Carolina;Klein, Jon;Filler, Renata B.;Strine, Madison S.;Sy, Mouhamad;Deme, Awa B.;Badiane, Aida S.;Dieye, Baba;Ndiaye, Ibrahima Mbaye;Diedhiou, Younous;Mbaye, Amadou Moctar;Diagne, Cheikh Tidiane;Vigan-Womas, Ines;Mbengue, Alassane;Sadio, Bacary D.;Diagne, Moussa M.;Moore, Adam J.;Mangou, Khadidiatou;Diallo, Fatoumata;Sene, Seynabou D.;Pouye, Mariama N.;Faye, Rokhaya;Diouf, Babacar;Nery, Nivison;Costa, Federico;Reis, Mitermayer G.;Muenker, M. Catherine;Hodson, Daniel Z.;Mbarga, Yannick;Katz, Ben Z.;Andrews, Jason R.;Campbell, Melissa;Srivathsan, Ariktha;Kamath, Kathy;Baum-Jones, Elisabeth;Faye, Ousmane;Sall, Amadou Alpha;Velez, Juan Carlos Quintero;Cappello, Michael;Wilson, Michael;Ben-Mamoun, Choukri;Tedder, Richard;McClure, Myra;Cherepanov, Peter;Some, Fabrice A.;Dabire, Roch K.;Moukoko, Carole Else Eboumbou;Ouedraogo, Jean Bosco;Boum, Yap, II;Shon, John;Ndiaye, Daouda;Wisnewski, Adam;Parikh, Sunil;Iwasaki, Akiko;Wilen, Craig B.;Ko, Albert, I;Ring, Aaron M.;Bei, Amy K. - 通讯作者:
Bei, Amy K.
Impact of strategies to mitigate misinformation in diverse settings and populations: a protocol for a living evidence synthesis.
- DOI:
10.1136/bmjopen-2023-076672 - 发表时间:
2023-10-12 - 期刊:
- 影响因子:2.9
- 作者:
Wilson, Michael;Velez, Marcela;Lavis, John - 通讯作者:
Lavis, John
Influence of middle hepatic vein resection during right or left hepatectomy on post hepatectomy outcomes.
- DOI:
10.14701/ahbps.21-159 - 发表时间:
2022-08-31 - 期刊:
- 影响因子:0
- 作者:
Nutu, Anisa;Wilson, Michael;Ross, Erin;Joshi, Kunal;Sutcliffe, Robert;Roberts, Keith;Marudanayagam, Ravi;Muiesan, Paolo;Chatzizacharias, Nikolaos;Mirza, Darius;Isaac, John;Dasari, Bobby V. M. - 通讯作者:
Dasari, Bobby V. M.
High risk injection drug use and uptake of HIV prevention and treatment services among people who inject drugs in KwaZulu-Natal, South Africa.
- DOI:
10.1371/journal.pone.0281030 - 发表时间:
2023 - 期刊:
- 影响因子:3.7
- 作者:
Zanoni, Brian;Milford, Cecilia;Sithole, Kedibone;Mosery, Nzwakie;Wilson, Michael;Bosman, Shannon;Smit, Jennifer - 通讯作者:
Smit, Jennifer
Wilson, Michael的其他文献
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{{ truncateString('Wilson, Michael', 18)}}的其他基金
Dissecting mechanisms of gene control in the vertebrate acute phase response
解析脊椎动物急性期反应中基因控制的机制
- 批准号:
RGPIN-2019-07041 - 财政年份:2022
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Dissecting mechanisms of gene control in the vertebrate acute phase response
解析脊椎动物急性期反应中基因控制的机制
- 批准号:
RGPIN-2019-07041 - 财政年份:2021
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Dissecting mechanisms of gene control in the vertebrate acute phase response
解析脊椎动物急性期反应中基因控制的机制
- 批准号:
RGPIN-2019-07041 - 财政年份:2020
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Dissecting mechanisms of gene control in the vertebrate acute phase response
解析脊椎动物急性期反应中基因控制的机制
- 批准号:
RGPIN-2019-07041 - 财政年份:2019
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
- 批准号:
436194-2013 - 财政年份:2018
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
- 批准号:
436194-2013 - 财政年份:2017
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
- 批准号:
436194-2013 - 财政年份:2016
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
- 批准号:
436194-2013 - 财政年份:2014
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
Developing a multi-species platform for understanding mammalian gene control
开发了解哺乳动物基因控制的多物种平台
- 批准号:
436194-2013 - 财政年份:2013
- 资助金额:
$ 2.55万 - 项目类别:
Discovery Grants Program - Individual
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