Characterization and mitigation of experimental and natural viral bioaerosols

在过去的十年里，我们已经获得了关于呼吸道病毒分子本质的非常详细的知识， 我们对它们的传播方式知之甚少解决这一问题对于制定循证战略至关重要 以防止这些病毒的传播。新型呼吸道病毒的出现，如H7N9禽流感病毒 和中东呼吸综合征冠状病毒（MERS-CoV）突出了识别手段的重要性， 这是为了控制传染性生物气溶胶，以减少对加拿大公众的风险。10年前， 死亡和重大破坏;恐慌和警报源于缺乏对这种传播方式的了解 新型病毒不幸的是，这一问题至今仍未解决。 我们已经确定了重要的知识差距，我们建议开发采样工具并评估各种方法，以减轻 自然和实验气溶胶的扩散。我们与两家加拿大公司和公共卫生部合作， 加拿大卫生署，以确保有关呼吸道病毒传播的知识有效地转移到应用部门。 我们还将利用我们建立的流感病毒体外和体内（雪貂）传播模型， 作为最先进的气溶胶表征系统，以评估一种新的病毒气溶胶检测仪器， Airmax我们还将使用这些系统来评估传统杀菌方法的新应用， 脉冲紫外线技术这些发现将对我们如何应用感染预防产生重大影响 以及季节性和新出现/重新出现的呼吸道病毒的控制措施，同时为 控制这些代理人。

We have gained extraordinarily detailed knowledge in the past decade about the molecular nature of respiratory viruses, yet we know relatively little about how they spread. Resolution of this issue is essential for developing evidence-based strategies to prevent transmission of these viruses. The emergence of novel respiratory viruses such as the H7N9 avian influenza virus and the Middle East Respiratory Syndrome coronavirus (MERS-CoV) highlights the importance of identifying means by which to control infectious bioaerosols in order to reduce risk to the Canadian public. A decade ago SARS-CoV caused death and significant disruption; panic and alarm stemmed from the lack of knowledge of the mode(s) of transmission of this novel virus. Unfortunately, this question remains unresolved today. We have identified important knowledge gaps and we propose to develop sampling tools and evaluate methods to mitigate the dispersion of natural and experimental aerosols. We have partnered with two Canadian companies and the Public Health Agency of Canada to ensure efficient knowledge transfer about respiratory virus transmission into the applications sector. We will utilize our established in vitro and in vivo (ferret) transmission models for influenza virus, as well as state-of-the-art aerosol characterization systems to evaluate a new viral aerosol detection instrument developed with Airmax. We will also use these systems to evaluate a novel application of a traditional germicidal approach that utilizes pulsed ultraviolet developed by Topnotch. These findings will have a significant impact on how we apply infection prevention and control measures for seasonal and emerging/re-emerging respiratory viruses, whilst informing public health policy for the control of these agents.