Exploring PFpP organometallic nanoparticles for sensing technology

探索用于传感技术的 PFpP 有机金属纳米颗粒

• 批准号: 479146-2015
• 负责人: Wang, Xiaosong
• 金额: $ 1.82万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579962
• 关键词: Exploring; PFpP; organometallic; nanoparticles; sensing

Environmental Bio-Detection Products Inc. (EBPI) in Mississauga is developing sensing techniques for monitoring of a range of environmental contaminants. In order to develop low cost, but highly sensitive and robust sensing devices, techniques to collect analytes effectively and concentrate to sufficient levels to allow detection down to the ug/L range are crucial and remain a significant challenge. Aqueous colloids assembled from metal carbonyl organometallic polymers, PFpP, possess large surface areas per unit mass and offer the promise to serve as ideal materials for sample/analyte collection if their surface can be successfully decorated with analyte binding groups. A second step will require the concentration of these particles containing the bound analytes from a range of complex environmental matricies, e.g. natural waters. This concentration step will be necessary to allow for the detection of the concentrated analytes at environmentally meaningful levels. To address EBPI's specific problems, involving analyte collection and concentration, the PFpP colloids will be decorated with mamaleimide groups. This will in turn allow RNA aptamers to be tethered via thiol-maleimide reactions to the MC colloids. The tethered aptamers are able to complementary bind to specific DNA for target detection. In addition, by taking advantage of the hollow structure of the MC colloids, magnetic nanoparticles will be encapsulated within them. The resultant hybrid particles, after collecting analyses, can be concentrated using magnetic forces allowing for high detection of specific target analytes at environmentally meaningful concentrations (ng to ug/L levels) . Dr. Don Lush with a background in environmental biology, and Dr. Aaron Witham with a background in molecular chemistry, employees of EBPI will participate in directing the research toward rapid initial product commercialization.

环境生物检测产品公司（EBPI）在密西索加正在开发用于监测一系列环境污染物的传感技术。为了开发低成本、高灵敏度和耐用的传感装置，有效收集分析物并浓缩至足够水平以允许检测低至微克/升范围的技术至关重要，并且仍然是一项重大挑战。 由金属羰基有机金属聚合物PFpP组装的水性胶体具有每单位质量大的表面积，并且如果其表面可以成功地用分析物结合基团修饰，则其有望用作样品/分析物收集的理想材料。第二步将需要从一系列复杂的环境基质（例如天然沃茨）中浓缩这些含有结合的分析物的颗粒。该浓缩步骤是必要的，以允许在环境有意义的水平上检测浓缩的分析物。 为了解决EBPI的具体问题，包括分析物的收集和浓缩，PFpP胶体将被修饰mamaleimide基团。这将反过来允许RNA适体通过硫醇-马来酰亚胺反应与MC胶体连接。拴系的适体能够互补地结合至用于靶检测的特异性DNA。此外，通过利用MC胶体的中空结构，磁性纳米颗粒将被封装在其中。在收集分析后，可以使用磁力浓缩所得的混合颗粒，从而允许在环境有意义的浓度（ng至ug/L水平）下高度检测特定目标分析物。具有环境生物学背景的Don Lush博士和具有分子化学背景的Aaron威瑟姆博士，EBPI的员工将参与指导 快速的初步产品商业化的研究。