Developing RHCC-NT as new monitoring and remediation tool for Poly-Aromatic Hydrocarbons (PAHs)

开发 RHCC-NT 作为多环芳烃 (PAH) 的新监测和修复工具

• 批准号: 479210-2015
• 负责人: Stetefeld, Jörg
• 金额: $ 14.14万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Strategic Projects - Group
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=579989
• 关键词: Developing; RHCC; NT; new; monitoring

The economic benefit from the Oil & Gas industry is significant to Canada and has to be sustainable in the long-term. It is incumbent to ensure that the environment is not negatively impacted. Polycyclic aromatic hydrocarbons (PAHs) are naturally occurring constituents of crude oil and are generally categorized as saturated acyclic and cyclic carboxylic acids. PAHs are quite corrosive and are removed from crude oil (e.g., bitumen) during its extraction from oil fields and oil sands, and during subsequent refining of the recovered crude oil. The consequence is that water used for recovery and processing of crude oil becomes increasingly enriched with PAHs. Significant amounts of dissolved PAHs remain in waste process water and consequently, are stored in large tailings ponds. The problem with PAHs is that their elevated levels in natural waterways are extremely toxic to aquatic life including bacteria, fish, plants and mammals. Furthermore, it is possible for these pollutants to move from the natural waterways into municipal water supply systems. In collaboration with STANTEC, we are proposing to develop a right-handed coiled coil nanotube (RHCC-NT) based passive sampler device (PSD) that detects and/or recovers PAHs from fluid and sediment systems. The nanotube is based on the RHCC domain from Staphylothermus marinus (S. marinus), an extremophillic archea bacterium isolated from black smoker vents. As a consequence of its adaptation to the extreme conditions at the deep sea volcano, the S-layer protein is characterized by an unusual stability (pH, Temperature, pressure, salinity, ionic strength) that makes it a perfect tool for biotechnological developments. In this project, we aim to develop novel technologies based on RHCC-NT PSDs that can detect fast and in the sub-nM range diverse priority PAH's with the goal to establish a cost effective monitoring device and to establish a new generation bio-availability tool. In addition, we will extend the unique storage capacity of large-sized cavities to develop RHCC-NT as a PAH-remediation tool. Such technologies will significantly improve the water quality systems in Canada and benefit our economy through enhanced environmental sustainability.

石油和天然气行业的经济效益对加拿大来说非常重要，并且必须是长期可持续的。有责任确保环境不受负面影响。多环芳烃（PAH）是原油中天然存在的组分，通常分为饱和无环羧酸和环状羧酸。多环芳烃具有很强的腐蚀性，可以从原油中去除（例如，在从油田和油砂中提取沥青的过程中，以及在随后的回收原油的精炼过程中，其结果是，用于原油回收和加工的水越来越富含多环芳烃。大量溶解的多环芳烃残留在废水中，因此储存在大型尾矿池中。多环芳烃的问题是，它们在天然水道中的含量升高，对水生生物，包括细菌，鱼类，植物和哺乳动物具有极强的毒性。此外，这些污染物有可能从天然水道进入城市供水系统。与STANTEC合作，我们建议开发一种基于右手螺旋线圈纳米管（RHCC-NT）的被动采样器装置（PSD），用于检测和/或回收流体和沉积物系统中的多环芳烃。该纳米管是基于来自海洋葡萄球菌（S。marinus），一种从黑烟囱喷口分离出的嗜极端古细菌。由于其适应深海火山的极端条件，S层蛋白质的特点是不寻常的稳定性（pH值，温度，压力，盐度，离子强度），使其成为生物技术发展的完美工具。在这个项目中，我们的目标是开发基于RHCC-NT PSD的新技术，该技术可以快速检测亚纳米范围内的多种优先级PAH，目标是建立一种具有成本效益的监测设备，并建立新一代生物利用度工具。此外，我们将扩大大尺寸空洞的独特存储容量，以开发RHCC-NT作为PAH修复工具。这些技术将大大改善加拿大的水质系统，并通过加强环境可持续性使我国经济受益。