Non-invasive health assessment assay in sturgeon broodstock

鲟鱼亲鱼的非侵入性健康评估测定

• 批准号: 490949-2015
• 负责人: Fast, Mark
• 金额: $ 1.82万
• 依托单位: University of Prince Edward Island
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=586681
• 关键词: Non; invasive; health; assessment; assay

There are three species of sturgeon grown in culture for caviar within Canada. Over the past decade this industry has grown exponentially, and both Target Marine Hatcheries (BC) and Acadian Sturgeon and Caviar Inc. (NB),have experienced increased caviar production by >60% since 2011, and Breviro Caviar Inc. (NB) quadrupled its production in 2013 through a $2.5 million expansion (Financial Post, 2013). Now, Russian, Siberian and Beluga sturgeon are being marketed out of North Carolina and Florida, since 2014. Healthy, cultured fish are crucial to the profitability and sustainability of these industries, however, unlike terrestrial cattle, wildlife or even companion animal industries, fish culture and specifically sturgeon culture lacks any diagnostic test for infection, inflammation and or stress. As sturgeons are long lived, late maturing fish that do not spawn every year, and most of their populations suffer from extreme exploitation and endangerment, highly fecund broodstock are rare and extremely valuable. This creates an important niche for non-invasive, rapid health screening. In other species, acute phase protein (APP) assessments have become more valuable due to their sensitivity and predictive function. Serum amyloid A and haptoglobin (Hp) are highly conserved specifically and are known to respond in Atlantic sturgeon to bacteria/LPS with similar kinetics to higher vertebrates. Somru BioScience Inc. is an emerging biotechnology company dedicated to developing breakthrough antibody technology for research, diagnostic and therapeutic applications. Somru would like to initiate this project to establish the company as a high quality bioassay provider for the aquaculture industry. Somru develops custom antibodies and generate quality reagents that enable drug development and can perform fit-for purpose validations for antibody reagents. The objectives of this research are to: Determine conserved regions of APP (SAA, Hp, Srt) in sturgeon species, develop polyclonal antibodies to sturgeon APP and determine kinetics of APP in blood of Atlantic sturgeon under bacterial challenge.

加拿大境内养殖了三种用于制作鱼子酱的鲟鱼。在过去的十年里这 行业呈指数增长，目标海洋孵化场 (BC) 和阿卡迪亚鲟鱼和鱼子酱 Inc. (NB) 自 2011 年以来鱼子酱产量增加了 60% 以上，而 Breviro Caviar Inc. (NB) 通过 250 万美元的扩建，2013 年产量翻了两番（《金融邮报》，2013 年）。现在，俄语， 自 2014 年起，西伯利亚鲟和白鲟就在北卡罗来纳州和佛罗里达州销售。健康、 然而，与陆生鱼类不同，养殖鱼类对于这些行业的盈利能力和可持续性至关重要 牛、野生动物甚至伴侣动物产业、鱼类养殖，特别是鲟鱼养殖缺乏任何 感染、炎症和/或压力的诊断测试。由于鲟鱼是长寿的晚熟鱼类 并不是每年都会产卵，而且它们的大多数种群都遭受着极端的剥削和濒临灭绝，高度 多产亲鱼十分稀有且极其珍贵。这为非侵入性、快速的 健康检查。在其他物种中，急性期蛋白（APP）评估变得更有价值，因为 他们的敏感性和预测功能。血清淀粉样蛋白 A 和触珠蛋白 (Hp) 高度保守 已知大西洋鲟对细菌/LPS 的反应与较高浓度的细菌/LPS 具有相似的动力学。 脊椎动物。 Somru BioScience Inc.是一家新兴的生物技术公司，致力于开发 用于研究、诊断和治疗应用的突破性抗体技术。索姆鲁想要 启动该项目旨在将公司打造成水产养殖业的高质量生物测定提供商。 Somru 开发定制抗体并生成优质试剂，支持药物开发并可 对抗体试剂进行适合用途的验证。本研究的目标是：确定 研究鲟鱼物种中 APP (SAA、Hp、Srt) 的保守区域，开发针对鲟鱼 APP 的多克隆抗体 并确定细菌攻击下大西洋鲟血液中 APP 的动力学。