Role of BH3 only protien BNIP3 in regulating hypoxia stress responses.

BH3 唯一的蛋白质 BNIP3 在调节缺氧应激反应中的作用。

• 批准号: RGPIN-2015-06676
• 负责人: Gibson, Spencer
• 金额: $ 2.77万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591216
• 关键词: Role; BH3; only; protien; BNIP3

There is a balance between cell survival and death to maintain healthy organisms. As humans evolved, cells have developed protective strategies to prevent physiological stress from killing them. This could be during periods of low oxygen or starvation. In the brain, this protective method is essential to maintain normal functions. How the brain prevents against cell death is not completely understood. We are investigating a protein called BNIP3 that may act as a master controller for cell survival. When BNIP3 is expressed in high levels it promotes cell death. In contrast, when BNIP3 is found in the nucleus of brain cells, it leads to cell survival. BNIP3 can also be found in an alternative shorter form that promotes cell survival. We are investigating how BNIP3 is controlling the balance between cell survival and cell death through a process called autophagy. Autophagy is a process where the cell can recycle material to create nutrients and maintain cell survival. If autophagy is prolonged, it will lead to cell death. We will evaluate how the nuclear or short form of BNIP3 affect autophagy under physiological stresses. Next, we will define what other proteins bind to BNIP3 and what genes are involved leading to cell survival. This research will answer fundamental questions and provide discoveries that could lead to a new understanding of how cells survive physiological stresses.

细胞存活和死亡之间存在平衡，以维持健康的生物体。随着人类的进化，细胞已经发展出保护性策略，以防止生理压力杀死它们。这可能是在低氧或饥饿期间。在大脑中，这种保护方法对于维持正常功能至关重要。大脑是如何防止细胞死亡的还不完全清楚。我们正在研究一种名为BNIP3的蛋白质，它可能是细胞存活的主控制器。当BNIP3以高水平表达时，它会促进细胞死亡。相反，当BNIP3在脑细胞核中被发现时，它会导致细胞存活。BNIP3也可以以促进细胞存活的替代性较短形式存在。我们正在研究BNIP3是如何通过一个称为自噬的过程控制细胞存活和细胞死亡之间的平衡的。自噬是细胞可以回收物质以产生营养物质并维持细胞存活的过程。如果自噬延长，将导致细胞死亡。我们将评估BNIP3的核或短形式如何在生理应激下影响自噬。接下来，我们将确定哪些其他蛋白质与BNIP3结合，以及哪些基因参与导致细胞存活。这项研究将回答基本问题，并提供可能导致对细胞如何在生理压力下生存的新理解的发现。