Multimode Microplate Reader for Investigation of Biofilms and Design of Biosensing Assays

用于生物膜研究和生物传感检测设计的多模式酶标仪

• 批准号: RTI-2016-00152
• 负责人: Neethirajan, Suresh
• 金额: $ 8.01万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2015
• 资助国家: 加拿大
• 起止时间: 2015-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=591361
• 关键词: Multimode; Microplate; Reader; Investigation; Biofilms

Recent progress in understanding the complex molecular mechanisms of cells behavior has led to the development of novel technologies that have improved the overall quality of our life. This has been made possible by studying multiple aspects that determine a single biological phenomenon. The introduction of a state-of-the-art cell imaging multimode microplate reader will allow eight researchers of the University of Guelph to accelerate their studies and facilitate the development of life-changing biomedical technologies to enhance our health and the safety of our food. This instrument will be located in and managed by the University of Guelph's BioNano Laboratory of the School of Engineering, where it will be critical to support the investigation of pathogenic bacterial biofilms and development of biosensing assays. This indispensable tool will operate at maximum capacity for many years, supporting the research programs of several biological engineering researchers and also faculty members across 3 departments of the Ontario Veterinary College, Ontario Agricultural College and the College of Biological Science. The requested multimode microplate reader will not only replace the 10 year old equipment currently in the BioNano Laboratory, but will also add extra capabilities (i.e., automated digital microscopy, collection of fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence, bio-luminescence resonance energy transfer - BRET, TR-Fluorescence Resonance Energy Transfer - TR-FRET) to analyse cells under physiological conditions while offering the possibility to perform a variety of tests with only a single equipment instead of purchasing three different instruments. Training programs offered to students and technical personnel will maximize the return of this investment by significantly enhancing graduate research and education, and also by attracting new collaborations.

最近在理解细胞行为的复杂分子机制方面取得的进展导致了新技术的发展，这些技术提高了我们生活的整体质量。这是通过研究决定单一生物现象的多个方面而实现的。引进最先进的细胞成像多模式微孔板读卡器将使圭尔夫大学的8名研究人员能够加速他们的研究，并促进改变生活的生物医学技术的发展，以提高我们的健康和食品安全。该仪器将位于圭尔夫大学工程学院的生物纳米实验室并由其管理，在那里它将对支持病原菌生物膜的研究和生物传感分析的发展至关重要。这个不可或缺的工具将以最大容量运行多年，支持安大略省兽医学院，安大略省农业学院和生物科学学院三个部门的几位生物工程研究人员和教职员工的研究项目。所要求的多模微孔板阅读器不仅将取代BioNano实验室目前使用10年的设备，而且还将增加额外的功能(即自动数字显微镜，荧光强度，发光，荧光偏振，时间分辨荧光，生物发光共振能量转移- BRET，tr -荧光共振能量转移- TR-FRET)分析生理条件下的细胞，同时提供了只用一台设备而不是购买三种不同仪器进行各种测试的可能性。为学生和技术人员提供的培训项目将通过显著加强研究生研究和教育，以及吸引新的合作，使这项投资的回报最大化。