Using fission yeast to unravel polyadenylation-dependent gene regulation

使用裂殖酵母解开多腺苷酸化依赖性基因调控

• 批准号: 328374-2012
• 负责人: Bachand, Francois
• 金额: $ 2.91万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=595311
• 关键词: Using; fission; yeast; unravel; polyadenylation

Messenger RNAs (mRNA) are the fundamental molecules responsible for encoding cellular proteins. Because the balance between synthesis and decay determines the steady-state abundance of an mRNA, RNA degradation becomes an integral part of gene regulation. On the mRNA 3' end is a stretch of polyadenosine (A) that normally stimulates gene expression by promoting stability and cytosolic translation. Contrasting this canonical role of mRNA polyadenylation, the 3' end poly(A) tail can also represent a path to RNA destruction. Our research program is centered on understanding mechanisms by which polyadenylation controls mRNA stability in the fission yeast, Schizosaccharomyces pombe. Accordingly, we have recently discovered a polyadenylation-dependent mRNA degradation system that depends on a protein named, Mmi1, and that selectively degrades meiotic transcripts during the mitotic cell cycle. The objective of this proposal, which is a step in the pursuit of the long-term goal of this research program, is to determine the mechanism by which polyadenylation controls meiotic gene expression in fission yeast. The following specific aims are proposed:

信使rna （mRNA）是负责编码细胞蛋白质的基本分子。由于合成和衰变之间的平衡决定了mRNA的稳态丰度，RNA降解成为基因调控的一个组成部分。在mRNA 3'端是一段聚腺苷(a)，通常通过促进稳定性和细胞质翻译来刺激基因表达。与mRNA聚腺苷化的典型作用相比，3'端聚(A)尾部也可以代表RNA破坏的途径。我们的研究项目集中在了解多聚腺苷化控制分裂酵母（Schizosaccharomyces pombe） mRNA稳定性的机制。因此，我们最近发现了一种依赖于多聚腺苷化的mRNA降解系统，该系统依赖于一种名为Mmi1的蛋白质，并在有丝分裂细胞周期中选择性地降解减数分裂转录物。本研究的目标是确定裂变酵母中聚腺苷酸化控制减数分裂基因表达的机制，这是追求本研究项目长期目标的一步。提出了以下具体目标：