Using fission yeast to unravel co-transcriptional 3' end processing and transcription termination

使用裂殖酵母解开共转录 3 末端加工和转录终止

• 批准号: RGPIN-2017-05482
• 负责人: Bachand, Francois
• 金额: $ 3.64万
• 依托单位: Université de Sherbrooke
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=628953
• 关键词: Using; fission; yeast; unravel; co

3’ end processing is a fundamental step of gene expression that precedes RNA polyadenylation and that is intimately associated with transcription termination. 3’ end processing is a co-transcriptional process that involves the recruitment of RNA maturation factors by the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II (RNAPII). Despite a somewhat fair assessment of the players implicated in RNA processing, much is still unknown about the regulatory mechanisms underlying their integration with the transcription cycle. This is particularly true for 3’ end processing and transcription termination. Indeed, recent transcriptome-wide studies indicate that multiple polyadenylation sites (PAS) are used at most eukaryotic genes, as demonstrated in humans, mouse, plants, flies, and yeast. This process, known as alternative polyadenylation (APA), is emerging as a major layer of gene regulation, allowing the inclusion or exclusion of sequences that control the localization, stability, and translation of mRNAs. However, the mechanism of PAS recognition and how PAS selection is coordinated with transcription termination remains unclear. The

3'末端加工是基因表达的基本步骤，先于RNA多聚腺苷酸化并且与转录终止密切相关。3'末端加工是一个共转录过程，涉及RNA聚合酶II（RNAPII）最大亚基的羧基末端结构域（CTD）募集RNA成熟因子。尽管对参与RNA加工的参与者进行了某种程度上公平的评估，但关于它们与转录周期整合的调控机制仍有很多未知之处。这对于3'末端加工和转录终止尤其如此。事实上，最近的全转录组研究表明，多聚腺苷酸化位点（PAS）在大多数真核基因中使用，如在人类，小鼠，植物，苍蝇和酵母中所证明的。这一过程被称为选择性多聚腺苷酸化（阿帕），正在成为基因调控的一个主要层面，允许包含或排除控制mRNA定位、稳定性和翻译的序列。然而，PAS识别的机制以及PAS选择如何与转录终止协调仍不清楚。的