COVID-19: Inactivation of human coronaviruses in aqueous solutions using UV-C

COVID-19：使用 UV-C 灭活水溶液中的人类冠状病毒

• 批准号: 550720-2020
• 负责人: Aucoin, Marc
• 金额: $ 3.64万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Alliance Grants
• 财政年份: 2020
• 资助国家: 加拿大
• 起止时间: 2020-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=701754
• 关键词: COVID; 19; Inactivation; human; coronaviruses

There is significant potential that the novel coronavirus SARS CoV-2 (herein referred to simply as CoV-2) may be spread through inadequately treated water. The novel coronavirus has been detected by rtPCR in fecal samples and in municipal wastewater. Perhaps more concerning, in the 2003 outbreak of the closely related SARS coronavirus, there was documented transmission associated with sewage aerosols. These observations raise critical questions about the adequacy of treatment for drinking water and wastewater in Canada. Since treated wastewater is discharged into recreational waters including Lake Ontario, these questions must be addressed in order to protect public health. Ultraviolet (UV) is one of the primary disinfection techniques used for drinking water and wastewater in North America, but its efficacy against CoV-2 is unknown. In this work we are proposing to evaluate the susceptibility of coronaviruses. The work is split up into a surrogate stage and a pathogen stage. The surrogate stage will bookend the pathogen stage, with the first part of the surrogate stage preparing to enter the pathogen stage, and the latter part continuing the investigation on a broader panel of coronaviruses. In the first part, solutions containing human coronavirus 229E will be inactivated with different UV doses to obtain dose response curves in the Aucoin Lab at the University of Waterloo. The information on this virus is needed and will not only be used by Trojan but will also be communicated to the Public Health Agency of Canada. Once all protocols are established, testing will be moved to McMaster University, in collaboration with the Miller lab, to evaluate the susceptibility of CoV-2 (pathogen stage). Again this will directly inform the operation of UV reactors that operate across Canada for the treatment of water. In the last part of this project, the susceptibility of other coronaviruses, including OC-43, in different aqueous solutions and in the presence of different soiling agents will be evaluated.

新型冠状病毒SARS CoV-2（本文简称为CoV-2）可能通过未充分处理的水传播，这是很有可能的。已经通过rtPCR在粪便样本和城市污水中检测到新型冠状病毒。也许更令人担忧的是，在2003年爆发的密切相关的SARS冠状病毒，有记录的传播与污水气溶胶。这些意见提出了关于加拿大饮用水和废水处理是否充分的关键问题。由于处理后的废水被排放到娱乐沃茨，包括安大略，这些问题必须得到解决，以保护公众健康。紫外线（UV）是北美用于饮用水和废水的主要消毒技术之一，但其对CoV-2的有效性尚不清楚。 在这项工作中，我们建议评估冠状病毒的易感性。这项工作分为代理阶段和病原体阶段。代理阶段将为病原体阶段做好准备，代理阶段的第一部分准备进入病原体阶段，后一部分继续对更广泛的冠状病毒组进行调查。在第一部分中，将在滑铁卢大学的Aucoin实验室中用不同的紫外线剂量灭活含有人类冠状病毒229 E的溶液，以获得剂量响应曲线。我们需要有关该病毒的信息，这些信息不仅会被Trojan使用，还会传达给加拿大公共卫生局。一旦建立了所有方案，测试将转移到麦克马斯特大学，与米勒实验室合作，以评估CoV-2的敏感性（病原体阶段）。同样，这将直接通知在加拿大各地运行的紫外线反应器的操作，用于水处理。在该项目的最后一部分，将评估其他冠状病毒（包括OC-43）在不同水溶液中以及在不同污染剂存在下的敏感性。