Regulation of Androgen Metabolism in Frog Development

青蛙发育中雄激素代谢的调节

• 批准号: 418576-2012
• 负责人: Langlois, Valérie
• 金额: $ 2.33万
• 依托单位: Royal Military College of Canada
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=596417
• 关键词: Regulation; Androgen; Metabolism; Frog; Development

Environmental pollutants that affect reproduction are one of the contributing factors to the declining populations of amphibians worldwide. A better understanding of sex hormone synthesis in amphibians is critical to help discover the mechanisms of action of these pollutants. The objective of the proposed research program is to use known hormone synthesis inhibitors to further our understanding of the regulation and biological functions of enzymes and hormones involved in male endocrine signalling. The experimental approach includes the analysis of organismal, cellular and molecular endpoints and aims to correlate higher levels of biological organization, for example atypical histology, to subtle molecular changes (e.g., gene expression). Over the next five years, my research program will study testosterone metabolism during frog development and sexual maturity.

影响繁殖的环境污染物是全球两栖动物数量下降的原因之一。更好地了解两栖动物的性激素合成对于帮助发现这些污染物的作用机制至关重要。拟议的研究计划的目的是使用已知的激素合成抑制剂，以进一步了解参与男性内分泌信号的酶和激素的调节和生物学功能。实验方法包括生物体、细胞和分子终点的分析，目的是将更高水平的生物组织（例如非典型组织学）与细微的分子变化（例如，基因表达）。在接下来的五年里，我的研究计划将研究青蛙发育和性成熟过程中的睾丸激素代谢。