Analytical and toxicological evaluation of bioavailable naphthenic acids from oil sands process-affected water and groundwater using biomimetic extraction solid phase microextraction

采用仿生萃取固相微萃取对受油砂工艺影响的水和地下水中的生物可利用环烷酸进行分析和毒理学评价

• 批准号: 507076-2016
• 负责人: GamalElDin, Mohamed
• 金额: $ 1.82万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=610234
• 关键词: Analytical; toxicological; evaluation; bioavailable; naphthenic

Naphthenic acids (NAs) are a highly complex mixture of organic compounds naturally present in bitumen. Recent evidence suggests that NAs are among the most acutely toxic chemical classes present in oil sands process water (OSPW). Traditional analytical methods to characterize NA contamination are not suitable predictors of potential ecotoxicity because these methods fail to characterize the bioavailability of individual NA compounds. This project aims to develop a biomimetic extraction - solid phase microextraction (BE-SPME) method as a surrogate to estimate the toxicity of bioavailable NAs from OSPW and groundwater. The BE-SPME technique will be used as a tool to compare toxicity test results on the basis of system exposure (i.e., lipid uptake) rather than on external water concentrations. Gas chromatography flame ionization detection (GC-FID) and atmospheric pressure gas chromatography time-of-flight mass spectrometry (APGC-TOFMS) will be used to characterize the chemical profile of the bioavailable fraction. Microtox assay will be used to investigate the acute toxicity of the bioavailable fraction. Treated samples using physico, chemical and biological treatment processes (i.e., adsorption, ozonation and biological treatment) will be used to validate the method. By using this technique, the characterization of the bioavailable organic acids in both untreated and treated process water will be conducted, helping to increase our understanding of the potential impacts of treated process water on the environment. Overall, this work will support other studies focusing on effluent testing, field monitoring, site assessment and remediation by providing an improved screening tool for analytical and toxicological evaluation and will also be beneficial for the supporting organization - Imperial Oil - to provide a hazard assessment tool that may be used for risk assessment purposes.

环烷酸（NAs）是天然存在于沥青中的高度复杂的有机化合物混合物。最近的证据表明，NAs是油砂处理水（OSPW）中毒性最强的化学类别之一。表征NA污染的传统分析方法不适合预测潜在的生态毒性，因为这些方法不能表征单个NA化合物的生物利用度。本项目旨在开发一种仿生萃取-固相微萃取（BE-SPME）方法，作为替代方法来评估OSPW和地下水中生物可利用NAs的毒性。be - spme技术将被用作一种工具，根据系统暴露（即脂质摄取）而不是外部水浓度来比较毒性测试结果。气相色谱火焰电离检测（GC-FID）和大气压气相色谱飞行时间质谱（APGC-TOFMS）将用于表征生物可利用部分的化学特征。微毒素试验将用于研究生物可利用部分的急性毒性。使用物理、化学和生物处理过程（即吸附、臭氧化和生物处理）处理过的样品将用于验证该方法。通过使用该技术，将对未经处理和处理过的工艺水中的生物可利用有机酸进行表征，有助于增加我们对处理过的工艺水对环境的潜在影响的理解。总的来说，这项工作将通过为分析和毒理学评价提供一种改进的筛选工具来支持其他侧重于污水测试、现场监测、现场评估和补救的研究，也将有利于支持组织——帝国石油公司——提供一种可用于风险评估目的的危害评估工具。