Microbial Adaptation and Evolution Towards Host Specificity

微生物对宿主特异性的适应和进化

• 批准号: RGPIN-2016-06196
• 负责人: Neumann, Norman
• 金额: $ 2.26万
• 依托单位: University of Alberta
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616154
• 关键词: Microbial; Adaptation; Evolution; Towards; Host

The bacterium, Escherichia coli, is part of the normal microbial flora found in the intestines of a variety of animals and the environment. Certain strains are known to cause disease in both animals and humans. It is generally believed that E. coli can transmit between different animals and is therefore considered to be a host-generalist. However, an increasing body of literature demonstrates that strains of this bacterium are very different at the genetic level, suggesting that some strains may be better adapted to survive within certain animal hosts or environments and, consequently, may have limited ability to cross into different animal populations (i.e., they may be host-specific). Recent research in my laboratory supports this concept. The research presented in this proposal attempts to identify novel genetic biomarkers of E. coli that define which animal host(s) or environments the organism may be capable of colonizing. The research is based on the hypothesis that E. coli’s survival within the gut of a particular animal host or environment will depend on its ability to sense and respond to the physiological gut conditions of that host or environment (i.e., water or wastewater), and consequently, whether it can outcompete other microbes for the limited nutrients/replication sites within the niche. Since gut and environmental conditions vary widely from host-to-host and from environment-to-environment, I believe that microbial evolution will drive survival strategies towards host specialization. The ability of the bacteria to ‘sense-and-respond’ to environmental conditions in the gut/environment is largely encoded in the ‘sensory genome’ (i.e., transcriptome) and consequently an analysis of DNA sequences in these control regions may reveal DNA biomarker patterns that correlate with host-specificity. As a corollary hypothesis I believe that intergenic DNA sequences from E. coli in humans, animals and the environment are 'imprinted' with host specific information, useful for identifying the source of emergent pathogenic strains of E. coli in food and water. To accomplish this, my laboratory will: a) mass sequence targeted areas of the genome (already shown by our group to carry host-specific biomarkers) across 5000 animal E. coli isolates, and b) use whole genome sequencing of representative animal isolates (supplemented with publically available genome data for human and animal isolates) to identify host-specific biomarkers using novel logic regression statistical tools developed by team members. The research is important in identifying disease-causing strains that have the potential to emerge from one animal host into another, or in the case of situations where zoonoses emerge (i.e., E. coli O104 in Europe), what animal reservoirs may have been associated with emergence. The data may also allow for development of control measures related to contamination of food and/or water.

这种细菌名为大肠杆菌，是在各种动物和环境的肠道中发现的正常微生物菌群的一部分。已知某些菌株会在动物和人类中引起疾病。一般认为，大肠杆菌可以在不同的动物之间传播，因此被认为是一种宿主通才。然而，越来越多的文献表明，这种细菌的菌株在遗传水平上非常不同，这表明一些菌株可能更适合在某些动物宿主或环境中生存，因此，它们进入不同动物种群的能力可能有限(即，它们可能是宿主特有的)。我的实验室最近的研究支持这一概念。这项提案中提出的研究试图确定新的大肠杆菌遗传生物标记物，这些标记物定义了该生物体可能能够定植的动物宿主(S)或环境。这项研究基于这样的假设，即大肠杆菌在特定动物宿主或环境的肠道内的生存将取决于它对宿主或环境(即水或废水)的生理肠道条件的感知和反应能力，从而决定它是否能够在生态位内有限的营养/复制位置上与其他微生物竞争。由于肠道和环境条件因宿主和环境的不同而有很大差异，我相信微生物进化将推动生存战略朝着宿主专门化方向发展。细菌对肠道/环境中环境条件的‘感觉和反应’能力在很大程度上编码在‘感觉基因组’(即转录组)中，因此，对这些控制区的DNA序列的分析可能会揭示与宿主特异性相关的DNA生物标志物模式。作为一个推论，我相信来自人类、动物和环境中的大肠杆菌的基因间DNA序列‘印记’了宿主特有的信息，有助于识别食物和水中新出现的致病菌株的来源。为此，我的实验室将：a)对5000个动物大肠杆菌分离物的基因组目标区域(我们小组已经证明携带宿主特异性生物标记物)进行大量测序，以及b)使用具有代表性的动物分离物的全基因组测序(辅以公开可用的人和动物分离物的基因组数据)，使用团队成员开发的新的逻辑回归统计工具来识别宿主特异性生物标记物。这项研究对于确定有可能从一种动物宿主传播到另一种动物宿主的致病菌株，或者在出现人畜共患病的情况下(即欧洲的O104大肠杆菌)，哪些动物宿主可能与出现相关，具有重要意义。这些数据还可用于制定与食品和/或水污染有关的控制措施。