Atmospheric Pressure Gas Chromatograph and Ultra-Cold Freezer to Support an Existing Ion Mobility Quadrupole Time of Flight Mass Spectrometer

支持现有离子淌度四极杆飞行时间质谱仪的大气压气相色谱仪和超冷冷冻机

• 批准号: RTI-2017-00131
• 负责人: Stark, Ken
• 金额: $ 6.89万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2016
• 资助国家: 加拿大
• 起止时间: 2016-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=616566
• 关键词: Atmospheric; Pressure; Gas; Chromatograph; Ultra

Mass spectrometry techniques are critical to identify specific molecular structures and to quantitate specific compounds. Dr. Ken Stark has recently added an ultra-high performance liquid chromatograph (UHLPC) Ion Mobility Quadrupole Time of Flight Mass Spectrometer (IM-QTOF) to the University of Waterloo Mass Spectrometry Facility (UWMSF). This instrument set up is capable of high sensitivity due to high resolution and accurate mass along with additional ion mobility separations. With UHPLC, this instrument is well designed for discovery lipidomic, proteomic and metabolomic research of relatively stable compounds. An atmospheric pressure ionization and gas chromatograph (APGC) package is available for this instrument that allows for rapid switching between front-end APGC and UHPLC. The addition of APGC would allow the IM-QTOF to be used for the analysis of a much broader range of compounds. Specifically, APGC is ideal for the introduction of volatiles and non-polar compounds such as fatty acids and various environmental contaminants, that can be problematic for UHPLC. The APGC also utilizes “soft” ionization which preserves the structure of the compound to allow for ultra-trace quantitation which is currently difficult with the current infrastructure in the UWMSF. This instrument would also enable the research programs of several other researchers at Waterloo, as they would be able to use the IM-QTOF to examine small volatile compounds at the core of their research programs. This grant also includes a request for an ultra-cold freezer. The increase in instrumentation and increased users requires that samples, especially biological samples be stored prior to analyses. Ultra-cold freezers are necessary to maintain the integrity of the samples and the target analytes.

质谱技术对于鉴定特定分子结构和定量特定化合物至关重要。Ken Stark博士最近为滑铁卢大学质谱设施（UWMSF）增加了一台超高效液相色谱仪（UHLPC）离子迁移四极杆飞行时间质谱仪（IM-QTOF）。由于高分辨率和准确的质量沿着加上额外的离子迁移率分离，该仪器设置能够具有高灵敏度。该仪器采用UHPLC，专为发现相对稳定化合物的脂质组学、蛋白质组学和代谢组学研究而设计。大气压电离和气相色谱仪（APGC）包可用于该仪器，允许在前端APGC和UHPLC之间快速切换。APGC的加入将使IM-QTOF能够用于更广泛的化合物分析。具体而言，APGC是引入挥发物和非极性化合物（如脂肪酸和各种环境污染物）的理想选择，这些化合物可能会对UHPLC造成问题。APGC还利用了“软”电离，保留了化合物的结构，以允许超痕量定量，这在UWMSF目前的基础设施中是困难的。该仪器还将使滑铁卢其他几位研究人员的研究计划成为可能，因为他们将能够使用IM-QTOF来检查其研究计划核心的小挥发性化合物。这笔赠款还包括一个超冷冰箱的请求。仪器的增加和用户的增加要求在分析之前储存样品，特别是生物样品。超低温冷冻箱是保持样品和目标分析物完整性所必需的。