Thermal cycler and nanodrop spectrophotometer

热循环仪和纳米滴分光光度计

• 批准号: 390238-2010
• 负责人: Stark, Ken
• 金额: $ 2.18万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443140
• 关键词: Thermal; cycler; nanodrop; spectrophotometer

Genetically manipulated mice and quantitative real time polymerize chain (qRT-PCR) reaction analyses of the expression of the mRNA are important research tools and techniques for physiology research. These tools are critical for understanding the physiological processes at the molecular level. While these techniques and tools have been used for some time in biochemistry, biology and medicine, they are now being used in nutrition and kinesiology studies. Modern equipment to support these activities such as thermal cyclers and spectrophotometers are essential to support research activities, but also to enable hands-on training for undergraduate students. The amount of genetic and molecular based research as dramatically increased in Department of Kinesiology at the University of Waterloo largely as the result of the growth of new research programs. This has resulted in the addition of two genetically modified mouse colonies with two more colonies being developed. Maintenance of these colonies requires diligent genotyping. In addition efforts to determine the impact of nutrition and exercise at the molecular level require extensive examinations of gene expression that includes the use of qRT-PCR). Both genotyping and qRT-PCR require high capacity thermal cycling and spectrophotometry. The use of these techniques in research programs in the field also creates a need and rationale for introducing related training at the undergraduate level. The present equipment request will support the programs of three NSERC funded researchers and enable the undergraduate training in important molecular biology techniques.

基因操作小鼠和mRNA表达的定量真实的时间PCR（qRT-PCR）反应分析是生理学研究的重要研究工具和技术。 这些工具对于在分子水平上理解生理过程至关重要。 虽然这些技术和工具已经在生物化学，生物学和医学中使用了一段时间，但它们现在正在用于营养和运动学研究。 支持这些活动的现代化设备，如热循环仪和温度计，对于支持研究活动至关重要，但也可以为本科生提供实践培训。 滑铁卢大学运动机能学系基于遗传和分子的研究数量急剧增加，主要是由于新研究项目的增长。 这导致增加了两个转基因小鼠群体，另外两个群体正在开发中。 维持这些菌落需要勤奋的基因分型。 此外，在分子水平上确定营养和运动的影响需要对基因表达进行广泛的检查，包括使用qRT-PCR。 基因分型和qRT-PCR都需要高容量热循环和分光光度法。 在该领域的研究计划中使用这些技术也创造了在本科阶段引入相关培训的需要和理由。 目前的设备要求将支持三个NSERC资助的研究人员的计划，并使重要的分子生物学技术的本科生培训。