Multi-User Ultra High Performance Liquid Chromatography/Mass Spectrometry Instrument for High-Throughput Analysis

许多科学家需要分析和识别化学品和生物化学品，以支持具有广泛社会和环境目标的研究计划。随着新的和在某些情况下结构复杂的化学和生物化学材料的设计，制备和分离，需要更有能力的技术来鉴定它们。为了实现这一目标，不断发展的液相色谱和质谱领域提供了改进的分析模式，如通过最先进的设备所提供的。 请求提供资金，用于建立一个与能够碎裂的质谱仪（MS/MS）相连接的超高效液相色谱系统，以满足需要能够方便地监测化学反应和生物化学方案的进展和成功的研究界的实际需要。 所要求的仪器将为来自多个小组的研究人员提供第一个高通量手段，通过该手段来监测反应和方案，从而快速分析结果。所要求的仪器将取代目前使用的费力的工作台方法（TLC），从而使研究人员能够熟练掌握他们正在接受培训的行业中常规使用的技术。 所要求的多用户uHPLC/MS/MS仪器将位于达尔豪西化学质谱机构（DCMSF）。该设施已运营超过13年，其中共同申请人艾莉森·汤普森博士担任主任。该设施由一名专职的全职达尔豪西专家技术员提供支持，并提供专门的专门实验室空间和一间办公室。通过DCMSF提供的稳健、长期和成功的操作模型将被用作管理和维护所需uHPLC/MS/MS的一种手段;在此过程中，仪器将保持在研究就绪状态，供需要动手操作高通量和详细数据用于研究项目的学员使用。 申请是代表使用该设施的研究界提交的。在过去的四年中，该设施已满足了5个省15个机构的40多个研究小组的需求：所需的uHPLC/MS/MS将得到精心管理，以确保最大限度的使用，使用效率和最佳的研究洞察力。 所要求的uHPLC/MS/MS将使研究学员能够学习如何使用现代分析设备，以进一步实现以下领域的研究目标： ·为绿色技术发明改进的催化剂; ·探索新的结构多样性作为药物发现的工具; ·表征生物分子如蛋白质和氨基酸的分子行为; ·鉴定用于早期疾病检测的生物标志物; ·表征化学反应的机制;以及 ·合成新的功能材料。

Many scientists need to analyze and identify chemicals and biochemicals to support research programs with broad-ranging societal and environmental goals. As new and in some cases structurally complex chemical and biochemical materials are designed, prepared and isolated, more capable techniques are required for their identification. Towards this goal, the ever-evolving fields of liquid chromatography and mass spectrometry provide improved modes of analysis, as made available via state-of-the art equipment. Funds are requested for an ultra high performance liquid chromatography (UHPLC) system coupled to a mass spectrometer (MS) capable of fragmentation (MS/MS) to satisfy a hands-on research community in need of the ability to easily monitor the progress and success of chemical reactions and biochemical protocols. The requested instrumentation will provide researchers from multiple groups with the first high-throughput means by which to monitor reactions and protocols, and thus rapidly analyze outcomes. The requested instrumentation will replace laborious bench methods (TLC) currently in use, and will thus enable researchers to become experienced with techniques that are routinely used in the industries for which they are being trained. The requested multi-user uHPLC/MS/MS instrument will be located in the Dalhousie Chemistry Mass Spectrometry Facility (DCMSF). This facility has been operating for >13 years with Dr. Alison Thompson, a co-applicant herein, as the Director. The facility is supported by a dedicated full-time Dalhousie expert staff technician, as well as through the provision of dedicated specialized laboratory space and an office. The robust, long-term, and successful operating model made available through the DCMSF will be utilized as a means of managing and maintaining the requested uHPLC/MS/MS; in doing so the instrument will be maintained in a research-ready state for trainees needing hands-on high throughput and detailed data for their research projects. The application is submitted on behalf of the research community that uses the facility. Over the past four years the facility has served the needs of >40 research groups at 15 institutions in 5 provinces: the requested uHPLC/MS/MS will be carefully managed to ensure maximum usage, efficiency of use, and optimal research insight. The requested uHPLC/MS/MS will enable research trainees to learn how to use modern analytical equipment to further research goals in areas such as: • inventing improved catalysts for green technology; • exploring novel structural diversity as a tool for drug discovery; • characterizing the molecular behaviour of biomolecules such as proteins and amino acids; • identifying biomarkers for early disease detection; • characterizing mechanisms of chemical reactions; and • synthesizing new functional materials.