Eggshell membrane nano-particles (ESM-NPs) for biomedical applications

用于生物医学应用的蛋壳膜纳米颗粒（ESM-NP）

• 批准号: 514335-2017
• 负责人: Hincke, Max
• 金额: $ 4.15万
• 依托单位: University of Ottawa
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=619096
• 关键词: Eggshell; membrane; nano; particles; ESM

The chicken egg is a valuable nutritious food, and large markets exist for processed / purified egg yolk and egg white. However, more research is necessary to develop value-added applications for waste eggshell and eggshell membranes. Our proteomic analysis of eggshell membrane (ESM) has identified proteins with antimicrobial, immune-modulating, anticancer and antioxidant properties, and we have verified these activities in ESM. However, one of the biggest challenges for the use of ESM bioactive substrate is its poor solubility and bioavailability. These limitations could be resolved by reducing the size of the ESM particles to enhance the solubility and increasing the bioavailability to improve solubility. Nano/reduce sized ESM particles will enhance its dissolution and therapeutic efficacy against microbial infection, inflammation, and other disease conditions which were previously difficult to target due to size / solubility restrictions. The objective of the proposed study is to produce eggshell membrane nanoparticles (ESM-NPs) and evaluate its applications against cancer stem cells, microbial infection and inflammatory disease conditions, with an emphasis on skin applications. ESM-NPs will be produced from ESM and physically characterized. The antimicrobial activity of ESM-NPs will be evaluated by spread plate and microbroth dilution assay. ESM-NPs will be tested against human melanoma cell lines to evaluate its efficacy as an anti-skin cancer agent. ESM nanocream will be prepared and ESM-NP transport through cellulose acetate membrane and rat skin will be evaluated. The anti-inflammatory activity of ESM-NPs will also be evaluated. Studies with ESM will be extended to the nano-level, and will lead to the development of new high-value cosmetic and biomedical products from ESM by-products.

鸡蛋是一种有价值的营养食品，加工/纯化的蛋黄和白色有很大的市场。然而，还需要进行更多的研究，以开发废蛋壳和蛋壳膜的增值应用。我们对蛋壳膜（ESM）的蛋白质组学分析已经确定了具有抗菌，免疫调节，抗癌和抗氧化特性的蛋白质，并且我们已经验证了ESM中的这些活性。然而，使用ESM生物活性基质的最大挑战之一是其溶解性和生物利用度差。这些限制可以通过减小ESM颗粒的尺寸以提高溶解度和增加生物利用度以改善溶解度来解决。纳米/减小尺寸的ESM颗粒将增强其溶解和针对微生物感染、炎症和其他疾病状况的治疗功效，这些疾病状况以前由于尺寸/溶解度限制而难以靶向。拟议研究的目的是生产蛋壳膜纳米颗粒（ESM-NP）并评估其针对癌症干细胞、微生物感染和炎症性疾病的应用，重点是皮肤应用。将从无害环境管理中生产无害环境管理纳米颗粒，并对其进行物理表征。将通过涂布平板和微量肉汤稀释测定来评价ESM-NP的抗微生物活性。ESM-NP将针对人黑色素瘤细胞系进行测试，以评估其作为抗皮肤癌剂的功效。将制备ESM纳米载体，并评价ESM-NP通过醋酸纤维素膜和大鼠皮肤的转运。还将评价ESM-NP的抗炎活性。对无害环境管理的研究将扩展到纳米水平，并将导致利用无害环境管理副产品开发新的高价值化妆品和生物医学产品。